甘蓝显性雄性不育材料dgms793993不育性遗传效应分析及门纯合不育株的离体保存.docx

后未见带毒率增加。 后未见带毒率增加。 离体保存的甘蓝DGMS材料在保存过程巾虽然发生甲基化状态的改变以及TuMV病毒在甘蓝组 培材料体内存在，但尚未发现对组培材料的田『日J生长和植物学性状产生影响，这说明甘蓝课题组现 有的离体保存程序对甘蓝DGMS材料的保存是可行的。 5．在18．20℃下，研究了保存甘蓝纯合DGMS材料的适宜条件。单芽苇段适合042521．12的保 存，2-3芽筝殴适合03Z-OIZ608一I的保存。用封[J膜+塑料膜封u可减少042521．12和03Z．012608．1 的继代次数。03Z-012608．1在MS+6．BA0．2mg／L+NAA0．Img／L+甘露孵0．3．；}+活性炭0．02．j}+蔗糖3％ 的条件下，042521—12在MS+6-BA0．2mg／t，卜NAA0．Irag／L+蔗糖7％的条件下长期保存后存活率屉高。 保存后的材}：I转入新鲜的培养基中，98％以上的茎段均能恢复生长。 6．以042521—12，03Z-012608—1，IMZ．-012126-1三个不同球犁的甘蓝DGMS材料为试材，研究 了适合它们组培快繁的方法。适合042521．12快繁的培养基为：MS+6．BA0．5+NAA0．1+蔗糖3％、 pa6．1，适合03Z-Of7-608．1快繁的培养基为：MS+6．BA0．5+NAAo．I+蔗糖2．％、pH6．1，适合 04Z-012126-1快繁的培养基为：MS+6-BA0．5+NAA0．1+蔗糖2％，pH6．0；培养过程【{I封u膜作为封 口材料最佳。在相同的培养条件下，由于基冈型的不同增殖系数宵很大的芹别。圆球类犁的甘蓝 DGMS材料042521．12增殖系数晟高，尖球类掣的甘蓝DGMS材料03Z-012608．1增殖系数最低。 关键词：甘蓝，显性雄性不育，遗传效应，纯合不育株，离体保存 n AbstractThe Abstract The dominant genie male sterile DGMS 79—399-3 in cabbage(as follows abbreviating DGMS)has been successfully used in seed producion in China,but there still have some defects in practice．：(1)smile materials ale difficult to obtain the sensitive lnale sterile plants which occasionally produce few pollens。 it is difficu／t to obtain homozygo啷DGMS pI锄￡s(MsMs)：(2)the environmental sensitive trait of DGMS materials needs to be farther studied；(3)the genetic effects of sterile trait 111．Is t0 bc smdied；(4) the genetic stability of the homozygous DGMS materials and TuMV virus changes after longer／n vitro conservation；(5)the technology of in vitro conservation and proliferation for sonic DGMS genotypes need8 t0 be further improved．To conquer these obstacles，lots of DGMS cabbage as experimental materials．the following a∞as were studied：1．The relationship between the fertility of DGMS materials and the environment factors；2．The methods to induce and obtain homozygous DGMS materials；3．The genetic effectsoftheDGMS cabbage；4．Thedetectionofthegenetic stability andtheTuMV virus change during缸 vitro conservation of the DGMS cabbage；5．The methods of in vitro conservation and increasing pro