高浓度甘来油发酵1,3-丙二醇菌株的筛选及其发酵动力学研究.docxVIP

上海师范大学硕士学位论文 上海师范大学硕士学位论文 Astract Abstract 1,3-propanediol(1．3-PD)is acknowledged as one of six new petrochemical products curremly in the world．Its main function is an important monomer to synthesize a new type of polyester,polyether,polyurethane．1，3一Propanediol is produced by two ways，chemical synthesis and microbial conversion．It has advantages for high-converting enzyme and expression efficiency．Producing 1,3-propanediol by microbial fermentation have many obvious advantages and become the focuses of research．This article made the following findings： 1，Screening to tolerate high concentrations of glycerol 1,3一propanediol producing strain Screening of high concentrations of glycerol tolerant strains improve from a fmal glycerol concentration of 1 0％to 30％gradually，out of the low strain tolerance of glycerol，from the outset a high tolerance to strain glycerine．Furthermore inquiries may utilize glycerol microbial synthesis product，find a microorganism from glycerol and three found a strain capable of producing 1,3-propanediol(1abeled D1)，and thus its physiological and biochemical experiments and 1 6SrRNA homology phylogenetic analysis to determine the status of this strain came to the conclusion that it is KlebsieIla conclusions． 2，optimized production strains medium composition and main technical parameters Preliminary experiments using single factor optimization Klebsiella growth medium components and fermentation medium conditions were investigated in different components of the starter culture bacteria culture and fermentation of the product，and the use of response surface experimental design optimization the medium composition．Optimization of growth medium were：KH2P04 1．49 g／L，K2HP04 3．4 g／L，(NH4)2S04 3 g／L，CaCl20．02 g／L，FeS04 1．3 g／L，yeast extract，1 g ／L，glycerol 50．3 g／L，for the optimization of fermentation mediumKH2P04 1．5 g／L，K2HP043．4