基于抗生素基因为报告基因的核酸酶效力检测-药理学专业论文.docxVIP

英文摘要基于抗生素基因为报告基因的核酸酶效力检测 英文摘要 基于抗生素基因为报告基因的核酸酶效力检测 Evaluation of Gene Editing Enzymes’ Acting Based on ZeocinR Reporter Gene Abstract Objective: To develop new assays for the evaluation of the enzymatic activities and off-target effects of gene editing enzymes. Methods: Bleomycin (zeocin) resistance gene (ZeoR) was chosen as the target of a new system for the evaluation of the enzymatic activities and off-target effects of gene editing enzymes. A prokaryotic vector possessing double antibiotic activities, AmpR-ZeoR was first constructed. An enzyme site of BaeI that is compatible to Golden gate