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SHORT COMMUNICATION doi:10.1111/j.1365-2052.2009.01954.x
Genetic variation and relationships among Turkish water buffalo
populations
† ¨ †
M. Gargani*, L. Pariset*, M. I. Soysal , E. Ozkan and A. Valentini*
*Department of Animal Production, University of Tuscia, Viterbo, Italy. †Department of Animal Science, Namik Kemal University, Tekirdag,
Turkey
Summary The genetic variation and relationships among six Turkish water buffalo populations,
typical of different regions, were assessed using a set of 26 heterologous (bovine) micro-
satellite markers. Between seven and 17 different alleles were identified per microsatellite in
a total of 254 alleles. The average number of alleles across all loci in all the analysed
populations was found to be 12.57. The expected mean heterozygosity (HE) per population
ranged between 0.5 and 0.58. Significant departures from Hardy–Weinberg equilibrium
were observed for 44 locus–population combinations. Population differentiation was
analysed by estimation of the FST index (values ranging from 0.053 to 0.123) among
populations. A principal component analysis of variation revealed the Merzifon population
to show the highest differentiation compared with the others. In addition, some individuals
of the Danamandira population appeared clearly separated, while the Afyon,
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