厚朴枝中厚朴酚及和厚朴酚提取工艺比较.PDF

32 2010, Vol. 31, No. 06 食品科学 ※工艺技术 张朝晖，王秋兰，姚茂君 (吉首大学化学化工学院，湖南吉首 416000) 采用加热回流提取法、超声波辅助提取法、微波辅助萃取法和超临界CO2 流体提取等技术从厚朴枝中提 取厚朴酚及和厚朴酚，优化提取条件，用反相高效液相色谱法(RP-HPLC)测定厚朴酚及和厚朴酚的提取得率，比 较不同提取方法对厚朴枝中厚朴酚及和厚朴酚的提取效果。结果表明，微波辅助提取厚朴枝中厚朴酚速度最快，当 微波功率为低火(1～2.8PV ，200W) 、乙醇体积分数65% 、料液比1:40(mg/mL)、提取时间10min 时，单次提取得 率最高，厚朴酚与和厚朴酚及总酚得率分别为7.893mg/g 和1.938mg/g。 厚朴酚；和厚朴酚；提取工艺；高效液相色谱 Comparative Study of Different Extraction Techniques for Extraction of Magnolol and Honokiol from Branches of Megnolia Hypoleuca ZHANG Zhao-hui ，WANG Qiu-lan ，YAO Mao-jun (College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China) Abstract：Heat reflux, ultrasonic-assisted extraction, microwave-assisted extraction and supercritical carbon dioxide extraction were compared for extraction of magnolol and honokiol from branches of Megnolia Hypoleuca . Reverse-phase HPLC was used for quantitative analysis of magnolol and honokiol. Results showed that among four extraction techniques, the least time was consumed for the microwave-assisted extraction of magnolol and honokiol, and the single microwave-assisted extraction for 40 min at 200 W (1 －2.8 PV) with 65% ethanol as extraction solvent using a material/liquid ratio of 1:40 (mg/mL) gave the highest yields of magnolol and honokiol, which were valued at 7.893 mg/g and 1.938 mg/g, respectively. Key words ：magnolol ；honokiol ；extraction technology ；high-performance liquid chromatography 中图分类号：R284.2 文献标识码：A 文章编号：1002-6630(2010)06-0032-04 厚朴为木兰科植物厚朴或凹叶厚朴的干燥根皮、枝 OH OH OH 皮及干皮，具有抗病毒、抗肿瘤、防龋齿、抗菌及 抗溃疡等作用[1