肠复康对人大肠癌HT229细胞增殖的影响及作用机制研究.pdf

肿瘤防治研究 2004 年第 31 卷第 12 期 · 957· 肠复康对人大肠癌 HT29 细胞增殖的影响及 作用机制研究 1 2 3 刘碧清 ,熊绍权 ,王柏丁 InfluenceandMechanismofChan gfukangonHumamLar geIntestineCancerHT 29 Cell Proliferation LIUBi qing,XIONGShao quan,WANGBai ding 1. Department of Tumor , Af filiated Hospital of Chengdu Universit y of Traditional Chinese Medicine , Chengdu 610072 , China ; 2. Department of Internal Medicine , 8670 Corps Hospital , Chinese People ’s Armed Police Force; 3. 2003 Grade of Doctoral Postgraduate , Clinical Medical College , Sichuan Uiniver sit y Abstract:Objective 　Toinvesti gatetheinfluenceofChineseCom poundReci peChan gfukangonhumamlar ge intestinecancercell proliferationandex ploreitsmechanism. Methods 　Modelnudemouseoftrans plantation tumorofhumanlar geintestinecancerHT 29wereestablished.Thelow,mediumandhi ghdose groupsof Changfukangweresetu p.Ei ghtweeksaftermedication,thetumorwei ght,inhibitionratesand patholo gic chan gesofeach groupwerecom pared.Thenumberof positiveKi 67cellsandtheintte grao pticaldensit yof vascularendothelial growthfactor (VEGF ) weresemi quantitativel ymeasuredb yimmunohistochemistr ystain methodscombinedwithima geanal ysiss ystem. Results 　Theinhibitionrateof 33.13% , 47.26% and 66.01% inthelow,mediumandhi ghdose groupsofChan gfukang.Thekar yon quantit yoftrans plantation tumorwasfewer,thecellandkar yonmor phologywasmorere gularineachChan gfukang group.Thenumber of positiveKi 67cellsandtheex pressionofvascularendothelial growthfactor (VEGF ) ineachChan gfukang groupwerealllowerthanthatintheblank group. Conclusion　Changfukanghasthefunctionofanti prolifer ationoftrans plantationtumorofhumanlar geintestinecancerHT 29,themechanismma ybecorrelatedwith its promotionofcancercelldifferentiationanditsinhibitionoftheex pressionofVEGF. Keywords: TraditionalChinesemedicine;Chan gfuka