补肾通络方与其灌胃大鼠血浆中活性成分测定方法分析.pdfVIP

ABSTRACT selectiveandsensitive objective：1．A highperformanceliquidchromatography was andvalidatedforthesimultaneousdeterminationof (HPLC)method developed inBushen Formula．2．Toestablish acid，stilbeneglucoside Tongluo loganin，feruilic anHPLCmethodfor and the acidandstilbene extractingdeterminingloganin，ferulic inrat afteradministeredwithBushen Formula．3．To glucosideplasmaig Tongluo a forthe ofSalviandicacidBinBushen establishAMSmethod semi-quantitative anHPLCmethodto TongluoFormula，and quantitative． were ona Methods：1．Chromatographicseparationssuccessfullyperformed C18 withamobile of stationaryphase phasecomposed with aceticacidataflowof1 mL／min．TheUVdetection 0．1％glacial wavelength Was a forthemaximum ofeach usingswitchingtechnology absorptionwavelength marked ofcolumnwas30。C．2．The was compounds．Thetemperature plasma methanoland extractedandtreatedwithamixtureof ethylacetate(3：l2V：V)，then extract to underastreamof theresidue plasma evaporateddryness nitrogenflow，and WasreconstitutedinHPLCmobileto conditionsof phaseinject．The chromatography： the Was onJohnson column C18