脑得生胶囊对家兔血管成形术后内皮功能和内膜增殖的影响.docVIP

脑得生胶囊对家兔血管成形术后内皮功能和内膜增殖的影响 冷吉燕 付军 吉林大学第一医院干部病房，吉林省长春市，130021 [摘 要]目的: 探讨脑得生胶囊对家兔血管成形术后内皮功能和内膜增殖的影响。方法:雄性大耳白兔30只，随机分成4组:对照组(n=6)，拉伤组(n=8)，高脂组(n=8)，脑得生组(n=8)。采用高胆固醇喂养加腹主动脉内皮剥脱制作血管狭窄模型，之后行血管球囊扩张成形术。于术后4周处死动物，取血管成形术部位血管标本，用免疫组化测定增殖细胞核抗原（PCNA）的表达水平。并分别于实验初、实验末兔耳缘静脉采血检测一氧化氮(NO)、内皮素（ET-1）浓度。结果:高脂组内膜增生最明显，内膜面积最大而管腔面积最小; 脑得生组内膜面积较高脂组明显减小，管腔面积明显扩大。高脂组血清总胆固醇（TC）、低密度脂蛋白（LDL-C）和ET-1均明显升高,而血清NO明显下降;脑得生组血浆TC 、LDL-C和ET-1明显降低,而血清NO水平明显升高。免疫组化染色:与高脂组比较，脑得生组PCNA的表达水平显著减少，差异具有显著性（p0.05）。结论: 脑得生胶囊具有抑制家兔血管成形术后内膜增殖和保护血管内皮功能的作用。 [关键词]：脑得生;血管成形术; 内皮功能;内膜增殖;PCNA The Inhibitory Effect of Naodesheng Capsule on the Endothelial Function and Intimal Proliferation after Angioplasty in Rabbits. Fu-Jun,Leng Jiyan Department of geraeology,The first hospital of Jilin university , Changchun, [Abstract] OBJECTIVE: To explore the inhibitory effect of Naodesheng Capsule on the endothelial function and intimal proliferation after angioplasty in rabbits. METHODS: Maleness adlut rabbit(n=30) were randomized into four groups, control group(n=6),injured group(n=8), hyperlipidemia group(n=8),Naodesheng Capsule group(n=8).Used high cholesterol diet and underwent denudation injury of abdominal aorta with balloon from femoral approach and a stent was implanted at stenosis segment when stenosis had formed angiographically. Animals in eachgroup survived for 4 weeks, The vessel samples from the site of angiop lasty were examined by immunohistochemical method to determinate the expression level of PCNA. And 吉林省中医药管局资助课题 冷吉燕，女，1964年生，吉林省长春市人，汉族，博士，硕士生导师，副教授，主要从事老年心脑血管病的诊治等方面的研究。E-mail：jiyanleng@.Telvenous blood was drawn from the ear of rabbits before and after experiment respectively to detected the contents of nitric oxide and in serum of endothelin in plasma.RESULTS: The results of optical microscopic examination showed there were significant difeernces in intimal area and luminal area between high-lipid group and naodesheng group.Compared with the control group the levels of serum total cholesterol