花生γ-生育酚甲基转移酶基因γ-TMT的克隆及序列分析-作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2012, 38(10): 1856−1863 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2012.01856 花生γ-生育酚甲基转移酶基因(γ-TMT) 的克隆及序列分析 * * 李拴柱 万勇善 刘风珍 / / , 271018 : γ- (γ-TMT)γ- α- , E PCR (A. hypogaea L.)4 γ-TMT DNA , AhgTMT ; 2 γ-TMT cDNA , AhrTMT AhgTMT AhrTMT , AhrTMT 1 059 bp, 352 AhTMT AhTMT 39.09 kD, 6.72, –0.12; α-55.40%, β-10.51%, 34.09%; , SAM AhTMT γ-TMT 61.75%~72.80%AhgTMT A. ipaensis (BB) A. batizocoi (BB)A. duranensis (AA)A. kuhlmannii (AA)4 100%99.91% 99.74%95.63% : ; E; γ- ; ; Cloning and Bioinformatic Analysis of γ-Tocopherol Methyltransferase Gene (γ-TMT) in Peanut * * LI Shuan-Zhu, WAN Yong-Shan , and LIU Feng-Zhen State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / Shandong Agricultural University, Tai’an 271018, China Abstract: γ-tocopherol methyltransferase(γ-TMT) is one of the critical enzymes that determine the composition and activity of vitamin E in plant. γ-TMT catalyzes the biological reaction from γ-tocopherol to α-tocopherol, is the most bioactive component of vitamin E. In this study, DNA sequence of γ-TMT in peanut (designated as AhgTMT ) was cloned by using in silico cloning com- bined with PCR from six cultivars and four diploid wild species that belong to Arachis section. cDNA sequence of γ-TMT (desig- nated as AhrTMT) was cloned from Fenghua 2. Nucleotide sequences of AhgTMT are identical with AhrTMT , which means no intron in thi