医疗机构血糖检测仪管理和临床操作规范.ppt

* * * * * 葡萄糖氧化酶 本张幻灯片显示葡萄糖氧化酶催化的反应。在检测时，GO催化从葡萄糖至铁氰化物的电子传递反应，生成亚铁氰化物。在检测末期，血糖仪在工作电极上加电压，驱动电子从亚铁氰化物流出，从而产生电流。血糖仪检测电流的强度进而据此计算出血糖的浓度。 Glucose Oxidase chemistry This slide shows the GO chemical reaction. During the test time, GO facilitates the transfer of electrons from glucose to ferricyanide, creating ferrocyanide. At the end of the test time, the Elite meter applies a potential to the working electrode, which draws electrons from ferrocyanide and creates a current. The meter measures the strength of electric current and calculates the glucose concentration. * GDH-PQQ 的化学反应 本幻灯片显示了PQQ的化学反应。在检测时，PQQ-GDH催化从葡萄糖至铁氰化物的电子传递反应，产生亚铁氰化物。在检测末期，血糖仪在工作电极上加电压，驱动电子从亚铁氰化物流出，从而产生电流。血糖仪检测电流的强度进而据此计算出血糖的浓度。 GDH-PQQ chemistry This slide shows the PQQ chemistry. During the test time, PQQ-GDH facilitates the transfer of electrons from glucose to ferricyanide, creating ferrocyanide. At the end of the test time, the ContourTM TS meter applies a potential to the working electrode, which draws electrons from ferrocyanide and creates a current. The meter measures the strength of electric current and calculates the glucose concentration. * GDH-FAD的化学反应 拜耳的试纸是一个应用以下方法的电化学系统：1）当血液进入检测室并溶解酶（葡萄糖脱氢酶）和反应中间体（铁氰化物）时，酶催化的葡萄糖传递电子给铁氰化物的反应就会开始，生成还原性的亚铁氰化物分子；2）血糖仪为工作电极和反电极之间加电压，导致铁氰化物又被氧化以及电子在电极之间流动。由此产生的电流被血糖仪检测并折算成葡萄糖浓度。 GDH-FAD chemistry Bayer’s test strip is an electrochemical system that employs the following method: 1) when blood enters the test chamber and dissolves the enzyme (glucose dehydrogenase) and the mediator (ferricyanide) a chemical reaction occurs in which the enzyme facilitates the passing of electrons from glucose to ferricyanide, creating the reduced molecule ferrocyanide; 2) the meter applies an electric potential between the working and counter electrodes, causing ferrocyanide to be oxidized and electrons to flow through the electrodes. The resulting current is measured and interpreted by the meter algorithm as glucose concentration. * 4种技术的优缺点. * * 内源性物质：