甘蔗种质资源遗传多样性研究.pdf

(2)RAPDof92 materials：50RAPD were analysissugarcanegermplasm primers to92tested materialsDNA totalof547bandswere amplified sugarcane samplesbyPCR，a was 10．9．Thereare530 andthe amplified，onaverage，eachprimeramplified polymorphic Was the 92 of rate to results．the 96．9％．According percentagepolymorphic clustering intotwo isdividedintotwosub materialsweredivided categories，eachcategory sugarcane totheresultsofRADP coefficient categories，according amplification，thesimilarity calculatedbetweenthe92cultivarsof resultsshowedthatthe sugarcane．The genetic coefficientfrom0．72to0．92．the coefficientbetweenTD8and similarity geneticsimilarity TD4is is0．92．The coefficientthevast ofmaterials highest，which similarity among majority is than0．7 greater (3) of resourcesbetweenUSAand Comparativeanalysissugarcanegermplasm domesticcultivars：28RAPD and8 ofSSR usedto25sugarcane primers pairs primers 1 RAPDwere 132 bandswere eultivars．The25 amplifiedbands，whichpolymorphic 02，and the of was77．3％．The bands foreach was percentagepolymorphic averageamplified primer 5．28bandsatotalof56bandswere SSR ofeac