夹竹桃内生真菌R22次生代谢产物的研究.pdf

关键词：夹竹桃，内生真菌，青霉属，5-羟基-8- 甲氧基-4-苯基-1 （2H ）异 喹啉酮，4- （2R, 4-二羟基丁氧基）苯甲酸，抑菌活性 II 万方数据 Studies of Secondary Metabolites from an Endophytic Fungi R22 of Nerium indicum ABSTRACT The research object of this experiment is the strain R22, which was isolated from the root of Nerium indicum with higher antimicrobial activities . The morphological characteristics and molecular biology characteristics revealed that the strain R22 is penicillium . The strain R22 was fermented by the rice solid medium. The loftdried culture was extacted with ethyl acetate and methanol to afford crude extract. The compounds were purified by silica gel column chromatography, gel column chromatography and recrystallization methods. Their structures were identified according to physicochemical property, spectrum data, high resolution mass spectrometry, infrared spectrum, ultraviolet spectrum and X-single crystal diffraction. Twenty compounds were identified as 26 alkyl acid (1), ergosterol (2), peroxy- ergosterol (3), 3-O-methylviridicatin (4), succinicacid (5), daturic acid-2,3-dihydroxypropylester (6), 3-methylquinazolin-4(3H)-one (7), 4-hydroxy -3,6-dimethyl-2-pyrone (8), viridicatol (9), cyclopenol (10), 5-hydroxy-8-metho- xy-4-phenylisoquinolin-1(2H)-one (11), daucosterol (12), 4-(2R, 4-dihydroxyb- -utoxy) benzoic acid (13), Aurantiomide C (14), arabite (15), Cyclo-(L-tryptoph- -yl-L-phenylalanyl) (16), mannitol (17), erythritol (18), allantoin (19), adenosine (20). 5-hydroxy-8-methoxy-4-phenylisoquinolin-1(2H)-one (11) and 4-(2R, 4-dihydroxybutoxy) benzoic acid