实用生物医学实验技术-Stem cell isolation cultutre and diff.pptx

干细胞分离、培养、与分化Stem cell isolation, culture, and differentiation;Prospective isolation of stem cells;Culture of stem cells;Differentiation of stem cells;I. MESENCHYMAL STEM CELL CULTURE:TOOLS AND TECHNIQUES;OVERVIEW;;SOURCES OF MSCs;CELL HARVEST;CELL HARVEST;CELL HARVEST;CELL HARVEST;PRIMARY CULTURE;PRIMARY CULTURE;PRIMARY CULTURE;CELL MORPHOLOGY;CELL PASSAGING;CRYOPRESERVATION; Adipose tissue represents abundant source of osteogenic precursors Easily obtainable Low donor morbidity ;ASCs ARE MULTIPOTENT;ADIPOGENIC DIFFERENTIATION;Chondrogenic differentiation media Dulbeccos Modified Eagle Med