英文文献汇报.pptVIP

Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies Xiaofei Li 1,2, Liting Qin 2, Haibo Zhu 3, Yingjun Sun 2, Xuezhi Cui 2, Yadong Gao 2, Xiaole Qi 1 , Yongqiang Wang 1 , Honglei Gao 1, Yulong Gao 1 , Xiaomei Wang 1 Arch Virol IF=2.058 Abstract Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can induce various clinical tumors. The capsid protein P27 is the group-specific antigen of ALV and has many viral antigen sites that are easy to detect. In this study, we produced a monoclonal antibody(mAb), 3A9, that is specific for the P27 protein. A series of partially overlapping peptides were screened to define 181PPSAR185 as the minimal linear epitope recognized by mAb 3A9. The identified epitope could be recognized by chicken anti-ALV and mouse anti-ALV P27 sera. The epitope was highly conserved among a number of ALV-A, ALV-B and ALV-J strains. MAb 3A9 might be a valuable tool for the development of new immunodiagnostic approaches for ALV, and the defined linear epitope might help further our understanding of the antigenic structure of the P27 protein. Avian leukosis viruses (ALVs), which belong to the family Retroviridae, are classified into A, B, C, D, E, and J subgroups[1, 2]. The A, B and J subgroups of ALV are the most common ALVs in commercial poultry, whereas subgroups C and D have rarely been reported. Subgroup E is a ubiquitous endogenous leukosis virus of low pathogenicity [3]. ALV predominantly causes lymphocytic leukemia, myeloid leukosis and other sarcomas and can also lead to immunosuppression effects, such as the abnormal development of immune organs, growth retardation, and decreased immune responses. [4, 5]. ALV subgroup J (ALV-J) was first isolated in the UK from white meat-type chickens in 1988 [2]. In China, ALV-J was first reported in 1999. Since then, ALV-J isolates have been detected in layer chickens, broiler breeders and local chickens throughout most reg