光动力疗法对根管内粪肠球菌感染的体外抗菌研究.doc

光动力疗法对根管内粪肠球菌感染的体外抗菌研究 王燕煌，郭晓霞，黄晓晶，雷丽珊 （350002 福州，福建医科大学口腔医学院牙体牙髓教研室；福建省高校口腔医学重点实验室） ?摘要? 目的 体外研究混合剂型亚甲基蓝光敏剂（Methylene Blue, MB）介导的光动力疗法（Photodynamic Therapy, PDT）对牛牙根管粪肠球菌（Enterococcus faecalis, E. faecalis）感染的抗菌效应。方法 体外选取30颗牛切牙样本建立E. faecalis根管感染模型，分别进行3种不同消毒方法，即单独超声冲洗，单独PDT处理及超声冲洗联合PDT处理。于根管消毒前、后取样，即刻培养检测根管内E. faecalis数目；实验结束后，每组随机选取3个样本扫描电镜（Scanning Electron Microscopy SEM）观察根管牙本质小管内残存细菌的形态。结果 3种消毒方法中，抗菌率最高为超声冲洗联合PDT处理组（99.92%），单独超声冲洗抗菌率高于单独PDT组（前者99.46%，后者98.3%），但二者相比较，差异无统计学意义（P 0.05）。SEM观察发现，3组细菌形态均发生变化，其中联合处理组变化最明显。结论 PDT作为根管消毒的辅助手段，配合超声冲洗能够提高抗菌疗效。 ?关键词? 亚甲基蓝；光动力疗法；粪肠球菌；超声冲洗；扫描电镜 ?中图分类号? R781.3; R454.2 The antimicrobial activity of photodynamic therapy against Enterococcus faecalis in vitro ? ?基金项目? 福建省自然科学基金项目资助（2010J01159） ?通讯作者? 黄晓晶，电话：0591E-mail：hxiaoj@163.com Wang Yanhuang, Guo Xiaoxia, Huang Xiaojing, Lei Lishan (Department of Endodontics, School of Stomatology, Fujian Medical University; State key Laboratory of Stomatology, Fuzhou, Fujian Province, 350002, China) ?Abstract? Objective To investigate the antibacterial activity of photodynamic therapy (PDT) against Enterococcus faecalis (E. faecalis) in infected bovine tooth model using methylene blue dispersed in a mixture. Methods The model of bovine root canals infected with E. faecalis was established which performed three different root canal disinfection measures. The control group was treated by ultrasonic irrigation with 2.5% NaOCl, while the other two test groups were treated by PDT or ultrasonic irrigation combined with PDT. Samples were collected from root canals immediately before and after disinfection to quantify the bacteria. After disinfection, three species per group were selected randomly and split longitudinally to examine the morphology of the residual bacteria inside the dentinal tubule with scanning electron microscopy (SEM). Results The best result in infected tooth model were formed by the combination groups which not only achieved 99.92% reduction but als