绿色木霉在啤酒糟基质上产纤维素酶条件的优化.pdf

2007,36(3):23-26. ( 363000) pH pH 5 628 30 4d 30% 50%(NH ) SO 2.0% 2.5% 4 2 4 TQ925 A 1009-7791(2007)03-0023-04 Studies on the Optimum Condition of Trichoderma viride Persoon ex fries Fermentation to Produce Cellulase CHEN Jian-xuan, CHEN Lian (Zhangzhou Vocational and Technical College, Zhangzhou 363000, Fujian China) Solid-state fermentation process was carried out to convert brewer’s grains to produce cellulase by Trichoderma viride Persoon ex fries. Such factors including initial moisture, fermentation time, initial pH value, the amounts of wheat bran and nitrogen source types were studied in this experiment. An optimum medium ingredient was obtained by primary test of single factor and orthogonal design experiment. The results were as follows. The best fermentation conditions were initial pH 56, fermentation temperature 28~30 and fermentation time 4d, and the best medium composition was wheat bran 30%, water contain 50% at least and (NH ) SO 2.5%. 4 2 4 Trichoderma viride; brewers; cellulase; optimum; condition 250kg [1-3] [4] 40% pH 2006-12-14 ZZY0625 1965- 24 36 1 1.1 Trichoderma viride 1.2 60 24h60 1.3 1.3.1 5 B 1.3.2 10%10g + 100ml 20min100ml5 B 11 1.3.3 24g6g(NH ) SO 2%30ml 4 2 4 1.4 1.4.1 50ml 500ml 115 15min 4d30 200r/min 3d 1.4.2 500ml 30g1ml 30 4d 1g 10ml 0.5mol/L 30 3h4 000r/min 15min 80 1.4.3 CMC-Na β-1,4