泡沫分离法提取竹节参皂苷工艺研究-中国试验方剂学杂志.doc

泡沫分离法提取竹节参皂苷工艺研究[收稿日期] [收稿日期] [基金项目] 国家自然科学基金项目 [第一作者] 张海滨，硕士生，从事中药化学及中药资源开发利用研究，Tel：0717－6397366，E-mail： 7765hui@163.com [通讯作者] * 袁丁， 教授，从事中药药理与中药化学研究，Tel：0717－6397366， E-mail：yxyyd@ 张海滨1，何毓敏1，张长城1，关乔中2，毛帅2，孙志伟1，袁丁1* （1?三峡大学医学院，湖北?宜昌?443002； 2?三峡大学化学与生命科学学院，湖北 宜昌 443002） [摘要] 目的:研究优化泡沫分离法提取竹节参皂苷的工艺条件。方法:分析提取物皂苷含量，优化泡沫分离的工艺条件。结果:当气泡直径在0.4-0.5mm、温度65℃、pH值5.6、电解质NaCl浓度为0.015mol/L时，总皂苷富集比为2.1，纯度比为2.6，回收率为98.33%。结论: [关键词] 泡沫分离; 竹节参皂苷; 富集比; 回收率; 纯度比 [中文分类号] R284.1 [文献标识码] A Technology research of Extracting saponins from Japanese ginseng using foam separation ZHANGHai-bin,HEYu-min,GUANQiao-zhong,MAO,Shuai,SUNZhi-wei,HU,ZHANGChang-cheng,YUAN Ding （1.Medical?College?of?China?Three?Gorges?University,?443002,Yichang?,China2. Chemistry And Bioscience College of?China?Three?Gorges?University443002 ,Yichang?,?China） [Abstract] Objective: to optimize the technological conditions that separated saponins of Panax japonicus by foam fractionation technique. Method: Orthogonal experiments analysis the content of the extraction from saponin, and thus optimize the foam separation process conditions. Results: Under the optimum conditions：the bubble diameter was 0.4-0.5mm, the temperature was 65℃, pH value was 5.6, and electrolyte NaCl concentration was 0.015mol/L as well, the enrichment ratio of total saponins was 2.1，purity ratio was above 2.6，and the recovery rate was 98.33%. Conclusion: Foam fractionation technique could be applied to separate total saponins from panax japonicas with its stability、r eliability and high yield, thus it provided a reference for the development and utilization of natural total saponins of Panax japonicas. [Key words] foam fractionation; saponins of Panax japonicas; enrichment ratio; purity ratio 泡沫分离是以气泡作分离介质, 利用被分离物质在气液两相界面吸附性质的差异来富集分离溶液中表面活性物质 [1]。皂苷类因具有一定表面活性，可被粘附在从溶液中升起的泡沫表面上，从而与母液分离[2]。 “土家族神药”竹节参近代药理研究学表明其主要化学成分为皂苷[3]，其分子结构中亲水性的糖基部分和亲脂的皂苷元所表现出的亲水性和亲脂性达到分子内动态平衡，具有表面活性[4]，而竹节参化学成分中的竹节参多糖、多种氨基酸、无机盐等不是表面活性物质，因此可以利用表面