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* * Knockout Mice The technique used to generate knockout mice was developed in the late 1980s by Marlo Capecchi at the University of Utah. 实验分三步∶ ◆构建重组体; ◆转基因敲除; ◆筛选。 Knockout mice Mario Capecchi (Late 1980s) (University of Utah) embryonic stem cells in inner cell mass as target cells 1/104 cells undergo a process of homologous recombination. Model organisms of study on cell biology 大肠杆菌与操纵子学说的建立及现代分子生物学的发展;豌豆和果蝇与遗传学定律的发现;酵母和海胆与对细胞周期调控机制的认识;线虫与对细胞凋亡机制的揭示;小鼠与哺乳动物功能基因组学的研究等等。 * * Exogenous and Endogenous RNAi Liposome-mediated Transfection * * Systems Biology of the Cell Currently, biologists tend to study the cell structure and behaviors as the ultimate objective to systemically understand the effect of — the molecules on the whole cell, and — the effect of the individual cell on the whole organism as well. cytomics * * * * E-cell (虚拟细胞 ) The computational model of living cell, reflecting the molecular networks within a cell. /software/e-cell-system E-Cell A concept of constructing virtual cells on computers. E-Cell Project An international research project aiming at developing necessary theoretical supports, technologies and software platforms to allow precise whole cell simulation. E-Cell System An object-oriented software suite for modeling, simulation, and analysis of large scale complex systems such as biological cells * * * * Cell Models for Research of Life Sciences Cell is the basic unit of life Cell has all the features of living things 3. Cell has whole inherit material 4. Cell has whole regulation on gene expression 5. The developments of modern techniques in researches THANKS! * Ell fusion 4. The Scanning probe microscope( SPM) –Scanning tunneling microscope(STM) Including: Scanning tunneling microscope (STM), Atom force microscope (AFM), Magnetic force microscope (MFM), Friction force microscope (FFM) STM的主要特点: (1)具有原子尺度的高分辨本领,侧
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