实验7 病毒形态学和培养及血清学English.ppt

Virus Demonstration Fungi : morphology, culture Laboratory diagnosis of viral diseases by morphological?method Morphology（electron microscope） Adenovirus, Herpesvirus CPE（cytologic examination） uninfected cells, infected cells Inclusion bodies of Rabies virus and Measles virus Operation Hemagglutination assay Hemagglutination inhibition test Video: Animal experiment Inclusion bodies Inclusion bodies are stainable structures which are either histologic changes in the cells caused by viral components or virus-induced changes in cell structures. They may appear within the nucleus or cytoplasm, and they can facilitate laboratory diagnosis Inclusion bodies of Rabies virus （Negri body） Negri body in a neuron Inclusion bodies of Rabies virus （Negri body） Inclusion bodies of Measles virus CPE Cell death Cell rounding, degeneration,aggregation,loss of attachments to the culture dish Charcteristic histologic changes Inclusion bodies, margination of chromatin Syncytia Cell surface changes Uninfected Vero cells CPE of HSV infection HSV-1-infected Vero cells VIRUS RBC Influenza virus Mumps virus Parainfluenza virus New castle disease virus Adenovirus Enterovirus Measle virus Reovirus Hemagglutination assay Hemagglutination inhibition test(HI Test) procedure for the measurement of serum antibodies directed against a hemagglutinating virus; the highest dilution of serum that completely inhibits hemagglutination by a standardized viral preparation is reported as the hemagglutination titer. Detection of antibody or serotyping of virus No. 1 2 3 4 5 6 7 8 9 10 Saline （ml） Virus （ml） 0.2 + 0.2 0.2 + 0.2 0.2 + 0.2 0.2 + 0.2 0.2 + 0.2 0.2 + 0.2 0.2 + 0.2 0.2 + 0.2 0.2 + 0.2 0.2 dilution 1∶10 1∶20 1∶40 1∶80 1∶160 1∶320 1∶640 1∶1280 1∶2560 control saline（ml） Each 0.2ml 0.5%RBC suspension（ml） Each 0.4ml Procedure of hemagglutination assay Label the wells from No.1 to 10. Add 0.2ml of saline to each wells. Dilute the virus according the table below. Add 0.2ml of sal