研究生抗体和细胞因子.pptx

Antibody and Cytokines 抗体和细胞因子 ANTIBODY and Immunoglobulin The structure and Function of Immunoglobulin (antibody) Ig genes and synthesis of protein Diversity of Ig and its mechanism Application of antibody Introduction Antibody (Ab) - protein produced by plasma cells in response to immunization and binds specifically to particular Ag. introduction Emil von Behring: The Founder of Serum Therapy Nobel Prize 1901 Paul Ehrlich:humour Immunity Nobel Prize 1908 Introduction Immunoglobulins (Ig) - The Igs are globulin which function as Abs or similar to Abs in chemical structure. - secreted Ig(sIg) ; membrane Ig(mIg) Rodney Porter 1917-1985 Nobel Prize in 1972 Gerald Edelman 1929 -2014 Nobel Prize in 1972 introduction Ig Ig 巯基乙醇 Heavy Chain Light Chain 1)Heavy Chain and Light Chain heavy chain (H chain) 50~75Kd 450~550aa H chain: μ γ α ε δ 1,structure 1)Heavy Chain and Light Chain light chain (L chain) 25Kd 214aa classes of L chain:   : 1~  4 structure 2.variable region and constant region variable region(V region) Light Chain - VL (110 aa) Heavy Chain - VH (110 aa) HVR or CDR FR HVR or CDR HVR: hypervariable regions CDR: the complementarity determining regions. CDR are found in both the H and the L chains. structure structure Framework regions FR (framework region) regions between the CDR in the V region are called the FR. VL and VH: Ag binding site structure Constant region(C) Light Chain - CL (110 aa) Heavy Chain - CH (330-440 aa) : CH1, CH2, CH3,( CH4) structure 3. Hinge Region - between the CH1 and CH2 region - IgM and IgE have no hinge region structure Domains - globular regions of 3D images of the Ig are called domains. Light Chain Domains VL , CL 2. Heavy Chain Domains VH, CH1,CH2, CH3 (or CH4) Domains of Ig Domains of Ig Domains of Ig J chain and