OIE《水生动物疫病诊断手册》 传染性肌肉坏死病 2018版2.2.5章只用条款4.3.1.2.3..pdfVIP

C H A P T E R 2 . 2 . 5 . I N F E C T I O N W I T H I N F E C T I O U S M Y O N E C R O S I S V I R U S 1. Scope Infection with infectious myonecrosis virus means infection with the pathogenic agent infectious myonecrosis virus (IMNV) that is similar to members of the Family Totiviridae. 2. Disease information 2.1. Agent factors 2.1.1. Aetiological agent, agent strains Phylogenetic analysis of its RNA-dependent RNA polymerase (RdRp) gene coding sequence indicates that IMNV is most closely related to Giardia lamblia virus, a member of the family Totiviridae (Fauquet et al., 2005; Lightner, 2011; Nibert, 2007; Poulos et al., 2006). IMNV particles are icosahedral in shape and 40 nm in diameter, with a buoyant density of 1.366 g ml–1 in caesium chloride. The genome consists of a single, double-stranded (ds) RNA molecule of 8226–8230 bp (Loy et al., 2015; Naim et al., 2015). Sequencing of the viral genome reveals two non-overlapping open reading frames (ORFs). The first ORF (ORF1, nt 470–5596) encodes a putative RNA-binding protein and a capsid protein. The coding region of the RNA-binding protein is located in the first half of ORF1 and contains a dsRNA-binding motif in the first 60 amino acids. The second half of ORF1 encodes a capsid protein, as determined by amino acid sequencing, with a molecular mass of 106 kDa. The second ORF (ORF2, nt 5884– 8133) encodes a putative RdRp (Poulos et al., 2006). The complete genomes of IMNV types originating from Brazil and Indonesia have been sequenced and found to be 99.6% identical at the nucleotide level (Poulos et al., 2006; Se