玉米生长调节因子(GRF)家族的全基因组鉴定及在非生物胁迫下的表达.doc

玉米生长调节因子(GRF)家族的全基因组鉴定及在非生物胁迫下的表达研究 赵训超，盖胜男，魏玉磊，许晓萱，丁冬，刘梦，张今杰，邵文静，徐晶宇*， （黑龙江八一农垦大学农学院，黑龙江省现代农业栽培技术与作物种质改良重点实验室，大庆，163319） 摘要：生长调节因子(GRF)在调节植物生长发育以及响应非生物胁迫扮演重要角色。本研究以拟南芥作为种子序列进行Blastp序列比对，共鉴定15个ZmGRFs基因并对其理化性质、系统进化关系、基因结构、保守基序、染色体定位、蛋白二级结构、磷酸化位点预测、各组织部位表达谱及在非生物胁迫下的表达进行分析。结果表明，根据系统发育树分析表明ZmGRFs基因与单子叶植物水稻亲缘关系较近；15个ZmGRFs基因分别定位在1、2、4、5、6、7、9和10号染色体上且各个基因之间高度同源；每个亚族之间基因结构及保守基序均相对保守；ZmGRFs蛋白二级结构均以α-螺旋和无规卷曲为主；根据磷酸化位点预测分析表明除ZmGRF6和ZmGRF9不含酪氨酸外，其它ZmGRFs蛋白均含有潜在的丝氨酸、苏氨酸和酪氨酸位点且各蛋白之间的潜在的磷酸化位点数目差异较大；根据转录组数据分析ZmGRF4和ZmGRF13基因在NaHCO3上调表达，同时发现热、盐和干旱胁迫下ZmGRF4和ZmGRF13基因表达较高；冷胁迫下ZmGRF1/3/4/15基因表达较高；根据不同组织部位表达谱分析显示ZmGRF4和ZmGRF13在各个部位表达均较高，以上结果为进一步明确GRF基因参与调节植物生长发育和非生物胁迫响应提供潜在的理论依据。 关键词：玉米(Zea may L)；生长调节因子(GRF)；非生物胁迫；表达分析；生物信息学 资助：黑龙江八一农垦大学优势特色学科建设项目专项资金(2042070002) *通讯作者：徐晶宇(Email:xujingyu2003@) Genome-wide identification and bioinformatics analysis of maize growth regulating factor (ZmGRFs) gene family and their expression profiles under abiotic stresses ZHAO Xun-chao, GE Sheng-nan, WEI Yu-lei, XU Xiao-xuan, DING Dong, LIU Meng, ZHANG Jin-jie, SHAO Wen-jing, XU Jing-yu* (College of Agronomy, Heilongjiang Bayi Agricultural University, Key Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Provinc, Daqing 16331) Abstract: Growth regulating factor (GRF) plays an important role in regulating plant growth development and responding to abiotic stress. In this study, Arabidopsis was used as seed sequence for Blastp sequence alignment. A total of 15 ZmGRF genes were identified and their physical and chemical properties, phylogenetic relationships, gene structure, conservative motifs, chromosome localization, protein secondary structure, prediction of phosphorylation sites, the expression profiles of different tissues and under abiotic stress expression were analyzed. The results show that according to phylogenetic tree analysis the relationship between GRF gene of maize and rice of monocotyledon plant was close; 15 ZmGRF genes were located on chromosomes 1,