皮肤鳞状细胞癌转录组学微阵列分析方法的研究策略及进展.PDF

Med J Chin PLA, Vol. 44, No. 6, June 28, 2019 　521 皮肤鳞状细胞癌转录组学微阵列分析方法的研究策略及进展 韩向春，郑力强，冯志芳，马静 [摘要]　皮肤鳞状细胞癌(cSCC)是角质形成细胞来源的恶性肿瘤之一。近年来，生物信息学方法在cSCC相关研究 中的应用非常广泛，包括基因组学、转录组学和蛋白组学等研究手段。其中转录组学是从整体水平研究基因的功能和 结构，揭示特定生物学过程中的分子机制，目前以微阵列(基因芯片)分析方法研究得较为深入。该文就转录组学研究 中比较常用的基因芯片表达数据库选择与处理流程，在线分析平台和相关软件，以及基因表达芯片在cSCC 中最新的研 究进展进行综述。 [关键词]　转录组学；微阵列；皮肤肿瘤；癌，鳞状细胞；研究策略；研究进展 [中图分类号]　R739.5　　　　[文献标志码]　A　　　　[文章编号]　0577-7402(2019)06-0521-06 [DOI]　10.11855/j.issn.0577-7402.2019.06.11 Research strategy and progression of microarray analysis in transcriptology of cutaneous squamous cell carcinoma 1 2* 2 2 HAN Xiang-chun , ZHENG Li-qiang , FENG Zhi-fang , MA Jing 1Department of Pathology, Tianjin Beichen Hospital of Traditional Chinese Medicine, Tianjin 300400, China 2Department of Dermatology, 81 Group Army Hospital of Chinese PLA Army, Zhangjiakou, Hebei 075000, China * Corresponding author, E-mail: zlqiang1976@163.com [Abstract]　Cutaneous squamous cell carcinoma (cSCC) is one of the malignant tumors originated from keratinocytes. In recent years, bioinformatics has been widely used in the research of cSCC, including genomics, transcriptionomics and proteomics. Transcriptionomics is to study the function and structure of genes at the overall level, and reveal the molecular mechanism of specific biological processes. At present, microarray (gene chip) analysis method has been studied deeply. The selection and processing of gene chip expression database, online analysis platform and related software, as well as the latest research progress of gene expression chip in cSCC have herewith been reviewed in present paper. [Key words]　transcriptionomics; microarray; skin neoplasms; carcinoma, squamous cell; research strategy; research progress 皮肤鳞状细胞癌(c u t a n e o u s