新生儿脐带血CD4+CD25high调节性T细胞Foxp3的表达.docVIP

DOC格式论文，方便您的复制修改删减 新生儿脐带血CD4+CD25high调节性T细胞Foxp3的表达 （作者:___________单位: ___________邮编: ___________） 作者：唐洁, 李柏青, 阮洁, 张林杰 【摘要】 　　目的: 检测新生儿脐带血CD4+CD25high调节性T细胞（Treg）数量及胞内转录因子Foxp3的表达, 探讨Treg细胞在新生儿期的表达特点。方法: 采集新生儿脐带血（n=15）和成人外周血（n=12）, 密度梯度离心法获取单个核细胞用荧光标记单克隆抗体（mAb）作表面和胞内染色后, 在流式细胞仪上检测CD4+CD25highTreg细胞的数量及其胞内转录因子Foxp3的表达。结果: 脐带血Treg细胞占CD3+CD4+T细胞的比例（3.86%±1.63%）明显高于成人外周血（0.87%±0.74%, P0.01); 而脐带血Treg细胞中表达Foxp3的比例明显低于外周血Treg细胞（23.21%±8.9% vs 71.3%±11.6%, P0.01）。结论: 虽然新生儿脐带血CD4+CD25highTreg细胞数量明显高于成人外周血, 但Foxp3+细胞数量明显低于成年人, 提示在功能上可能尚未成熟。 【关键词】 调节性T细胞 转录因子 新生儿脐带血 　　［Abstract］ AIM: To investigate the amount of CD4+CD25high Treg cells and the expression of Foxp3 in neonatal cord blood, and to analyze the expression of Treg cells in neonates. METHODS: The mononuclear cells from cord blood or peripheral blood were isolated from neonatal cord blood (n=15) or healthy adult’s peripheral blood (n=12) by density gradient centrifugation. Then they were stained with fluorescence labeled monoclonal antibodies for cell surface and intracellular protein. The amount of CD4+CD25high Treg cells and intercellular transcription factor Foxp3 were measured by flow cytometry. RESULTS: The amount of CD4+CD25high Treg cells in CD3+CD4+ T cells in cord blood was significantly higher than that in adult’s peripheral blood (3.86%±1.63% vs 0.87%±0.74%, P0.01), whereas the expression of Foxp3 in CD4+CD25high Treg cells in cord blood was markedly lower than in adult’s blood (23.21%±8.9% vs 71.3%±11.6%, P0.01). CONCLUSION: The low expression of Foxp3 in CD4+CD25high Treg cells in cord blood suggests Treg cells in neonates are not mature in suppression. 　　［Keywords］Regulatory T cell; transcription factor; neonate cord blood 　　1995年, Sakaguchi等［1］发现正常人外周血及脾脏的CD4+T细胞中存在着一类调节性T细胞, 特征性高表达IL-2受体a链（CD25分子）, 这种CD4+CD25+调节性T细胞（regulatory T cell, Treg细胞）在免疫应答的负调节及维持机体内环境稳定和诱导机体免疫耐受中发挥着重要作用。其后有研究结果显示在成人外周血中仅CD4+CD25high Treg细胞具有抑制功能［2］。近几年来, 许多研究表明转录因子Foxp3（forkhead box p3, Scurfin）在CD4+CD25highTreg细胞的发育和