植物组织培养教程李浚明2-3.pptVIP

* 第三节 植物离体培养体系的建立 一、植物组织培养的阶段划分： 通常将组织培养过程划分为四个阶段 1、起始培养阶段（诱导阶段） 2、增殖培养阶段 3、生根培养阶段 4、驯化移栽 Stage 0 – Selection preparation of the mother plant sterilization of the plant tissue takes place Stage I ?- Initiation of culture explant placed into growth media Stage II - Multiplication explant transferred to shoot media; shoots can be constantly divided Stage III - Rooting explant transferred to root media Stage IV - Transfer to soil explant returned to soil; hardened off Steps of Micropropagation 二、外植体起始培养（无菌体系建立） 起始培养阶段是指无菌体系的诱导和建立过程。主要受外植体、培养基、环境条