白介素6保护小脑颗粒神经元抵抗nmda的神经毒性作用.pdf

150 Acta Physiologica Sinica , April 25, 2007, 59 (2): 150-156 Research Paper Interleukin-6 protects cerebellar granule neurons from NMDA-induced neurotoxicity 1,2 1 1,* WANG Xiao-Chun , QIU Yi-Hua , PENG Yu-Ping 1Department of Physiology, Faculty of Medicine, and Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong University, Nantong 226001, China; 2Department of Microbiology and Immunology, School of Medical Technology, Jiangsu University, Zhenjiang 212023, China Abstract: Interleukin-6 (IL-6) is an important cytokine that participates in inflammation reaction and cell growth and differentiation in the immune and nervous systems. However, the neuroprotection of IL-6 against N -methyl-D -aspartate (NMDA)-induced neuro- toxicity and the related underlying mechanisms are still not identified. In the present study, the cultured cerebellar granule neurons (CGNs) from postnatal (8-day) infant rats were chronically exposed to IL-6 for 8 d, and then NMDA (100 μmol/L) was applied to the cultured CGNs for 30 min. Methyl-thiazole-tetrazolium (MTT) assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and confocal laser scanning microscope (CLSM) were used to detect neuronal vitality, apoptosis and dynamic changes of intracellular Ca2+ levels in the neurons, respectively. Anti-gp130 monoclonal antibody (75 ng/mL) was employed to the cultured CGNs with IL-6 to inhibit IL-6 activity so as to evaluate the role of gp130 (a 130 kDa glucoprotein transducing IL-6 signal) in mediating IL-6 neuroprotection. Western blot was used to measure the expressions of phospho-signal transducer and activator of transcription 3 (STAT3) and phospho-extracellular signal regulated k