CD133阳性_阴性肺癌细胞的分选、鉴定与差异基因的筛选.pdfVIP

CD133 阳性、CD133 阴性肺癌细胞的分选、鉴定及差异基因的筛选 结论 一、人肺癌组织中有 CD133 阳性细胞，数量稀少，呈小灶状分布。CD133 的表达水平与肿瘤组织学类型、肿瘤分级、临床分期无关。 二、采用磁式分选的方法成功从人肺腺癌A549 细胞株中分选出CD133 阳性 和CD133 阴性细胞。 三、体外实验证实CD133 阳性细胞能在无血清培基中生长并形成“sphere ”， 可在体外诱导分化，具有高增殖潜能，提示 CD133 阳性细胞具有肿瘤干细胞特 性，CD133 阳性细胞与阴性细胞生物学特性具有明显差异。 四、耐药基因芯片检测结果提示RARG 可能在CD133 阳性细胞耐药机制中 起重要作用。 五、转移基因芯片检测结果提示 CD133 阳性细胞的高增殖潜能及黏附性降 低、免疫逃逸可能在肿瘤转移过程中发挥主要作用；CD82 可能在CD133 阳性细 胞转移机制中起重要作用。 关键词：CD133 ；肺癌；磁式分选；基因芯片 5 CD133 阳性、CD133 阴性肺癌细胞的分选、鉴定及差异基因的筛选 Isolation and Identification of CD133 positive and CD133 negative cells in Human Lung Cancer ，and Screening of their Differential Genes Abstract Objective Drug resistance , metastasis and recrudescence of cancers lead to the failure of clinical treatment and death of patients with malignant tumors. Searching for genes associated with drug resistance and metastasis of tumor is very important for solving problems of clinical treatment, and play a key role in clarifying the mechanism of tumor metastasis and drug resistance. According to the background,we plan to isolate CD133+ cells and CD133- cells by using magnetic activated cell corting method.Then evaluate their bionomics by different methods . On this basis, we will screen the differential genes of cancer metastasis and drug resistance by DNA arrays. Methods 1.Immunohistochemical staining was used to detect the expression and distribution of CD133 in 81 cases of lung cancer and 10 cases of normal lung tissue. Then find out the relationships of CD133 expression and tumor histological type ,tumor