生物化学英文版biochemistrychapteraaandprimarystructureofproteinpart.pptVIP

3.9 Determining the Sequence  of Amino Acid residues Proteins can be sequenced in two ways: 1. Real amino acid sequencing 2. Sequencing the corresponding DNA in the gene Protein Sequencing Procedure in real amino acid sequencing 1. Determination of polypeptide chain number; 2. If more than one polypeptide chain, separate them; 3. Cleave (reduce) disulfide bridges; 4. Determine amino acid composition of each chain; 5. Determine N- and C-terminal residues; 6. Cleave each chain into smaller fragments by site-specific proteases or chemicals; 7. Determine the sequence of each chain by