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1. Microbial metabolic characteristics and ecological controlling in Petroleum Reservoir——A review Abstract : Petroleum reservoir has a variety of microbes with diverse metabolic characteristics and great variation. These microbes play an important role in geochemical cycle.Research on their metabolism and ecological relationships enables the understanding mechanism in Microbial Enhanced Oil Recovery(MEOR).Based on the present researches,we review the microbial metabolic characteristics and ecological relationships in oil reservoir. 1. 油藏微生物的代谢特征和生态结构调控 摘要:油藏环境中孕育着多种多样的微生物,这些微生物代谢类型多、变异性大,在微生物生态系统中占有重要位置。 研究油藏中微生物的代谢特征和相互之间的生态关系,有助于提升对微生物提高采收率机理的认识。本文对油藏环境中常见微生物类群的代谢特征与功能、生态结构与调控等进行了简要综述。 2. Abstract :[Objective] The aim of study was to investigate bacterial diversity of Populus Euphratica forest in the hinterland of Taklimakan desert.All the isolateds were be used as inoculants for silage and biofertilizer. [Methods]Strains were isolated by culture-dependent method.Gram staining,NaCl tolerance,enzyme activity (including amylase,esterase,cellulase) were determined by strand methods.Phylogenetic trees based on 16S rRNA gene sequences were constructed by using the neighbour-joining. [Results]A total of 27 strains were obtained.Phylogenetic analysis of the bacterial 16S rRNA gene showed that all isolates fell into one of the following four bacterial lineages:Actinobacteria(16 strains),Proteobacteria (4 strains),Firmicutes(6 strains) and Bacteroidetes(1 strains).Gram staining indicated that 5 strains were gram-negative and the others were gram-positive.Among these,15 strains showed amylase activity,9 strains showed esterase activity and 9 strains showed cellulase activity.All strains growth occurred at in presence of 2% NaCl,22 strains growth occurred at in presence of 5% NaCl and only 1 strain tolerated up to 15% NaCl.[Conclusion]The bacterial population diversity is abundant in soil of Populus Euphratica Forest in the hinterland of Taklimakan Desert. 2. 塔克拉玛干沙漠腹地胡杨林
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