Total Exosome RNA and Protein Isolation Kit - Life ：总RNA和蛋白质分离试剂盒外的生活.pdfVIP

user guide Total Exosome RNA and Protein Isolation Kit For isolation of rNA and protein from exosomes Catalog Number 4478545 Revision Date 28 June 2012 Publication Number MAN0006962 Contents Product Information 4 Product Description 4 Product Contents 6 Methods 7 Procedural Overview 7 Troubleshooting 13 Appendix A: Enriching for Small RNAs 15 Enrichment Procedure 15 Appendix B: Preparing RNA for Sequencing 17 Procedural Overview 17 (Optional) RNase III Fragmentation Procedure 18 Small RNA Library Construction Procedure 20 Perform Sequencing on the Ion PGM™ System 28 Appendix C: Western Blotting 29 Western Blotting Procedure 29 Appendix D: Isolation of RNA and Protein from Cells or Serum Samples 30 Introduction 30 Isolation of RNA and Protein from Cells 31 Isolation of RNA and Protein from Serum 34 Appendix E 35 Accessory Products 35 Technical Support 36 References 37 Purchaser Notification 38 3 Product Information Product Description Introduction The Total Exosome RNA and Protein Isolation Kit is designed for isolation of RNA and protein from a single enriched exosome preparation. Resuspended exosomes are divided, with one portion being used directly for common protein analysis applications such as western blotting, and the second proceeding to organic extraction and purification of total RNA. Maximal yields of ultra-