AlCl3抑制大鼠骨形茄成及BMPSmad信号转导通路的机制.docxVIP

优秀毕业论文 精品参考文献资料 CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese ． I Abstract in English ． ． ． ．．． ． ． ． ． ．． ． ．III 1 lnstrction ．．1 1．1 The toxicity ofAl bone l 1．2 Al and bone formation ．．2 1．2．1 Al and B．ALP ．2 1．2．2 Al and Col I ．3 1．2．3 Al and BGP ．．3 1．3 The effect ofAl BMP／Smad pathway inbone ．4 1．3．1 BMP一2 and bone formation 一4 1．3．2 BMP．2 and Smad family 4 1．3．3 BMP／Smad pathway and bone formation 4 1．zl Nuclear transcription factor and bone formation ．5 1．4．1 Runx-2 and bone formation ．．5 1．4．2 Osterix and bone formation ．．6 1．5 Researeh purpose and significance 6 2 Materials and methods． ． ． ． ． ． ． ． ．．7 2．1 Main equipments and reagents 7 2．1．1 Main equipments 7 2．1．：!Main reagents ．．7 2．2 The culture and treatment ofrat ．8 2．2．1 Experimental animal 8 2．2．2 The collection and treatment of rats ．．8 2．3 Detecting items and methods 8 2．3．1 The Al level in serum and bone 一8 2．3．2 The detection of BMD ．9 2．3．3 The detection of ALP，Coil，BGP,BMP-2，BMPR—IA，BM【PR-II，Smad4，Runx2，OSX mRNA expressions in bone ．．9 2．3．4 The detection of BMP-2，p-BMPRIA，p-SmadI／5／8 protein expressions in bone ．1 l 2-3．5 The effect ofAICl30n p—Smadl／5／8／4 protein expressions in nucleus ofbone 11 2．3．6 The effect ofAlCl30n Runx2，Osterix mRNA and protein expressions in bone ．12 2．4 Analysis of data and statistics 1 2 3 Results and Analy’sis 13 3．1 The Allevel in serum and bone 13 3．2 The effect ofAlCl3 BMD ．．13 3．3 The effect ofAICl3 B-ALP,BGP,Col I mRNA expressions in bone ．14 III 万方数据 东北农业大学农学硕上学位论文3 东北农业大学农学硕上学位论文 3．4 The effect ofAICl3 BMP-2，BMPR·IA，BMPR—lI，Smad4 mRNA expressions in bone 1 5 3．5 The effect ofAICl3 BMP一2，p-BMPRIA，p-Smadll5／8 protein expressions in bone 一17 3．6 The effect ofAICl30n p-Smadl／5／8／4 protein expressions in nucleus ofbone．．．．．．．．．．．．．．．．．．．．．1 8 3．7 The effect ofAICl30n Runx2，Osterix mRNA and protein expressions in bone ．1 9 3．7．1 The effect ofAICl30n Runx2．Osterix mRNA expressions in bone 一19 3．7．2 The effect ofAICl30n Runx2，Osterix protein exp