贵州省猪弓形虫血清学调查.docVIP

贵州省猪弓形虫血清学调查 洪尼宁，钱德兴，张登祥，华莹，李涛，冉隆仲，田海蓉，刘霞，虞天德 （贵州省动物疫病预防控制中心，贵阳550008） 摘要：为全面了解贵州省猪弓形虫感染情况，对采自9个市（州、地）261个养猪场（户）的2906份血清用酶联免疫吸附试验（ELISA）检测猪弓形虫抗体，总体阳性率为65.83％，变化范围为27.88～85.42%。采集65份猪血清做ELISA和间接血凝试验（IHA）比较测定，两种方法总体符合率为58.46%。用IHA方法补充检测177份猪血清，弓形虫抗体阳性率为27.68%。血清学调查表明猪弓形虫抗体阳性率与国内部分省（市）报道相符。 关键词：猪；弓形虫；血清学调查；阳性率 SEROLOGICAL SURVEY OF PORCINE TOXOPLASMA INFECTION IN GUIZHOU HONG Ni-ning, QIAN De-xing, ZHANG Deng-xiang, HUA Ying, LI Tao, RAN Long-zhong, TIAN Hai-rong, LIU Xia, YU Tian-de (Guizhou Provincial Center for Animal Disease Prevention and Control, Guiyang 550008, Abstract: To monitor the present status of porcine Toxoplasma infection in Guizhou province, a serologic survey was conducted. In this survey, 2906 swine sera were collected from 261 farms in 9 regions and tested for Toxoplasma antibody by using ELISA kit. The result showed that 65.83 % of the sera were positive with a fluctuation of 27.88～85.42%. 65 swine sera from a farm were examined comparatively by using ELISA and IHA (indirect hemagglutination assay) kits, which showed that the coincidence rate of the two methods was 58.46%. Additional 177 swine sera were tested with IHA and the positive rate was 27.68%. Analysis of the positive rate among several provinces or municipalities indicated that the seroprevalence of Toxoplasma antibody was coincident. Key words: swine; Toxoplasma; serologic survey; positive rate 作者简介 作者简介：洪尼宁（1968-），男，湖南人，博士，微生物学专业。E-mail: hongnn413@126.com，电话：0851-4822241，传真：0851-4846401 弓形虫病(Toxoplasmosis)是由刚地弓形虫(Toxoplasma gondii)引起的、包括人在内的350多种脊椎动物易感的寄生虫病[1],1908年Nicolle和Manceaux在北非鼠体、Splendore在巴西兔体中分别独立发现该寄生虫[2]。T. gondii是目前世界范围内视网膜感染的主要原因[3]，欧洲孕妇血清学调查证实30%–63%的弓形虫感染和食用猪肉有关[4]。近二十年来世界各地陆续发现和报告了大量临床及隐性感染的猪弓形虫病[5]，国内部分省（市）猪弓形虫抗体间接血凝试验（IHA）检测阳性率为29.70-66.7%[6-10]，酶联免疫吸附试验（ELISA）检测阳性率达60.42%［11］。为全面了解贵州省猪弓形虫感染的情况，我们对省内9个市（州、地）的养猪场（户）的猪进行了弓形虫血清学调查，同时对部分养猪场周边的牛、羊和鸡也进行了调查。 1 材料与方法  1.1 血清及诊断试剂和仪器 按常规方法采血、分离血清，-20℃储存。 动物弓形虫IgG抗体酶联免疫检测试剂盒，海泰生物制药有限公司生产，光吸收值（A）采用SUNRISE酶标仪（奥地利TECAN）获取。