牛蒡子提取物体外抑菌作用的研究-河南农业科学.pdf

DOI :10.15933/j.cnki .1004 -3268.2008.06.013 2008 年第6 期 ＊ 孙　佳, 王学英 , 臧　楠,石成林, 李　群, 陈　丹 ( , 110161) :用体积分数为70%的乙醇溶液提取牛蒡子,然后用滤纸片法测定提取液对病原菌的抑制作 用,用 倍稀释法确定提取液的最低抑菌浓度(MIC), 并进 一步研究提取液的热稳定性。结果表 明, 牛蒡子乙醇提取液对柞蚕链球菌、金黄色葡萄球菌、番茄早疫病菌和小麦赤霉病菌等有比较明 显的抑制效果, 而对玉米小斑病菌和玉米弯孢病菌没有抑制作用。提取液对柞蚕链球菌的最低抑 菌浓度为0.125 g/L,蜡质芽孢杆菌为0 .250 g/L, 苏云金芽孢杆菌、枯草芽孢杆菌、金黄色葡萄球菌 为0.500 g/L,乳酸菌为1.000 g/L,大肠杆菌、番茄早疫病菌为2 .000 g/L, 小麦赤霉病菌、柞蚕白僵 病菌、葡萄白腐病菌、谷子白发病菌、烟草赤星病菌、毛霉以及水稻恶苗病菌为4.000 g/L。热处理 对提取物的抑菌效果无显著影响(P0 .05)。 :牛蒡子;乙醇提取液;抑菌活性 + :S482.2 92　　:A 　　:1004-3268(2008)06-0074-04 A ntifungal Activity in v i tro of A rctium Ethanol E tract ＊ SUN Jia,WANG Xue-y ing ,ZANG N an,SHI Cheng-lin, LI Qun,CHEN Dan (College of Biotechnology, Sheny ang Agricultural University, Shenyang 110 16 1, China) Abstract:The antimicrobial activities of arctium e tract with 70%ethanol w ere studied in the paper.The result show ed that the e tract had significant antifungal effect against S tr ep tococcus p er ny i,S tap hy lococcus ureus ,A lter nar ia so lan i and F usari um g ramin earum ,but had no effect on H elmint hosp or i um may d is and Cur vu lari a luna ta .The M IC of e tract w as 0 .125 g/L to S tr ep to - coccus p erny i,0 .250 g/L to B aci l lus cer eus ,0 .500 g/L to B aci ll us thur ing iensis,B aci l lus cereus and S t ap hy lococcus ureus , 1.000g/L to L actobaci l lus acidop h i lus, 2.000 g/L to Escheri ch ia col i and A l ter nar ia solan i, and 4.000g/L to F usar i um g r aminear um ,B eauver ia bassi ana , Coni el la d ip lod iel la, S clerosp or a g r am in icol a, M ucor, A l ter nari a a l ter na ta and Cibberel la f uj ikuroi . Heat treatment had no signific