胶基型无烟气烟草制品中烟碱的释放行为研究-烟草科技.pdf

烟草科技/ Tobacco Science Technology 烟草化学/ Tobacco Chemistry 2013 年第6 期（总第311 期） 胶基型无烟气烟草制品中烟碱的释放行为研究 * 张文娟，何保江，屈 展，李 鹏 ，张建勋 中国烟草总公司郑州烟草研究院，郑州高新技术产业开发区枫杨街2 号 450001 摘要：研制了一种用于体外评价胶基型无烟气烟草制品烟碱释放行为的模拟咀嚼机，并利用该设备结合 高效液相色谱仪测试了4 种市售胶基型无烟气烟草制品的体外烟碱释放率。该设备主要包括溶媒存储 瓶、蠕动泵、恒温槽、循环水箱、载物台、空气压缩机、气缸、电机、气动夹子和释放液收集装置。其中循环 水箱和载物台形成的测试腔用来模拟人体口腔，以人工唾液为溶媒，空气压缩机带动气动夹子反复挤压 被测样品以模拟人体咀嚼状态。体内、体外烟碱释放测试结果表明：利用该模拟咀嚼机评价胶基型无烟 气烟草制品烟碱释放行为的方法简便可靠，测试结果与人体使用该类产品时的烟碱释放情况接近。该技 术对胶基型无烟气烟草制品的质量控制、烟碱生物利用度评价以及烟碱调控具有一定的应用价值。 关键词：胶基型无烟气烟草制品；烟碱；体内摄取率；体外释放；模拟咀嚼机 中图分类号：TS411.1 ，TS45 文献标识码：A 文章编号：1002-0861 （2013 ）06-0031-06 Evaluation of Release Behavior of Nicotine from Gum-based Tobacco Products * ZHANG Wenjuan, HE Baojiang, QU Zhan, LI Peng , and ZHANG Jianxun Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China Abstract: A simulated chewing machine was developed for evaluating invitro the release behavior of nicotine from gum-based smokeless tobacco products, and the in vitro nicotine release rates from four kinds of gum-based tobacco products on the market were tested by combining that machine with HPLC. The chewing machine was mainly composed of a container for solvent, two peristaltic pumps, a thermostat, two circulating water tanks, a sample stage, an air compressor, a cylinder, a motor, a pneumatic clamp and a liquid collector. The test chamber formed by the circulation tanks and sample stage simulated a buccal cavity, which ensured nicotine release at a constant temperature of 37 ºC into artificial saliva, the pneumatic clamp pushed by the air compressor compressed the test sample repeatedly simulating the chewing action. The in vivo and in vitro nicotine release tests showed that evaluating the release behavior of nicotine from gum-based tobacco p