体外模拟3种消化液对铁皮石斛多糖的消化作用.docxVIP

体外模拟3 种消化液对铁皮石斛多糖的消化作用张冠亚1，黄晓君1，聂少平1,*，崔武卫1,2（1.南昌大学 食品科学与技术国家重点实验室，江西 南昌 330047； 体外模拟3 种消化液对铁皮石斛多糖的消化作用 张冠亚1，黄晓君1，聂少平1,*，崔武卫1,2 （1.南昌大学 食品科学与技术国家重点实验室，江西 南昌 330047； 2.加拿大农业与农业食品部圭尔夫食品研究中心，安大略 渥太华 N1G 5C9） 摘 要：本实验采用人体胃肠道模拟系统，体外模拟3 种消化液对铁皮石斛多糖的消化行为。通过高效凝胶液相色 谱、铁氰化钾法和高效离子交换色谱分别测定消化后多糖分子质量变化、还原糖含量和单糖组成的情况。结果表 明：唾液不能改变铁皮石斛多糖的分子质量；模拟胃液和模拟胃肠液可以降低多糖的分子质量，同时提高多糖溶液 中的还原糖含量，但没有检测到单糖。结论：在体外模拟胃肠道的消化体系中，铁皮石斛多糖的分子质量减小，糖 苷键发生断裂，但没有游离单糖的释放。 关键词：铁皮石斛多糖；体外消化；分子质量；还原糖；单糖 Effects of Three Digestive Juices on the in vitro Digestion of Dendrobium officinale Polysaccharide ZHANG Guan-ya1, HUANG Xiao-jun1, NIE Shao-ping1,*, CUI Wu-wei1,2 (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2. Guelph Food Research Centre, Agriculture and Agri-Food Canada, Ottawa N1G 5C9, Canada) Abstract: The effects of three digestive juices on the in vitro digestion of Dendrobium officinale polysaccharide were investigated by using human gastrointestinal model system in vitro. By using high performance liquid chromatography (HPLC), potassium ferricyanide and high performance iron exchange chromatography (HPIEC), we determined the changes in the molecular weight, reducing sugar content and monosaccharide composition of the polysaccharide after digestion. Results showed that the digestion in saliva did not change the molecular weight of Dendrobium officinale polysaccharide. The digestion in simulated gastric juice and gastrointestinal juice reduced the molecular weight, and increased the reducing sugar content, but no monosaccharides were detected in the digested solution. Conclusion: After digestion in simulated gastrointestinal system, the molecular weight of Dendrobium officinale polysaccharide was reduced and the glycosidic bond was broken, but without releasing free monosaccharide. Key words: Dendrobium officinale polysaccharide; in vitro digestion; molecular weight; reducing sugar; monosaccharides 中图分类号：TS201.2 doi:10.7506/spkx1002-6630-201423054 文献标志码：