临床少见致病酵母的致病力和体外药物敏感性及电泳核型的初步研究.docx.pdfVIP

雷公藤内酯醇对体外培养的成骨细胞增殖的影响 雷公藤内酯醇对体外培养的成骨细胞增殖的影响 中华皮肤科杂志 2000 年第 2 期第 33 卷 论著 作者：黄岚 冯树芳 王洪复 金慰芳 魏道林 高建军 单位：黄岚 冯树芳（ 200040 上海医科大学华山医院皮肤科） ；王洪复 金慰芳 魏道林 高建军（上海 医科大学放射医学研究所） 关键词：雷公藤内酯；成骨细胞 【摘要】 目的 观察雷公藤内酯醇 （TL）对体外培养成骨细胞增殖的影响， 以进一步探讨雷公藤致女性骨质 疏松的机制。方法 以 Sprague?Dauley(SD)大鼠成骨细胞增殖为模型，分离新生 SD 大鼠头盖骨成骨细胞， 传代后加入不同浓度 TL。每浓度组设 4 个复孔，以 PBS作阴性对照，用 MTT 比色分析法检测 TL 在体外对 成骨细胞增殖的作用和效价。结果 培养 2d ， 10ng/mLTL 显著抑制成骨细胞增殖 (P＜ 0.01) ；培养 4d ， 0.01ng/mLTL 显著抑制成骨细胞增殖 (P＜0.01) 。药物对成骨细胞增殖的抑制程度与其剂量呈正相关 (P＜0.05) 。 结论 TL 微量时即显著抑制成骨细胞增殖，且呈剂量依赖性。雷公藤对成骨细胞的直接抑制作用是其导致 药物性骨质疏松的一个重要原因。 Effects of Triptolide on the Proliferation Capacity of Osteoblasts in vitro HUANG Lan, FENG Shufang, WANG Hongfu,et al. （Department of Dermatology, Huashan Hospital, Shanghai Medical University ， Shanghai 200040） 【 Abstract 】 Objective The effects of Triptolide （ TL） on the proliferation capacity of osteoblasts were studied in order to further explore the mechanism of osteoporosis induced by Tripterygium Wilfordii Hook.F (TW). Methods Osteoblasts were obtained from the calvaria of new? born SD rats and were cultured with various concentrations of TL. The effects of TL on proliferation capacity of osteoblasts were detected by MTT methods. Results The proliferation rates of osteoblasts cultured for 2 days and 4 days were significantly inhibited by 10 ng/mL and 0.01 ng/mL of TL respectively. The inhibition rate was positively correlated with TL concentrations. Conclusion It is indicated that the direct suppressive effects of TL on proliferation capacity of osteoblasts play an important role in the pathogenesis of osteoporosis induced by TW. 【 Key words 】Triptolide Osteoblast 雷公藤因其具有抗炎、 免疫抑制、 抗肿瘤及调节环核苷酸代谢等作用而广泛应用于临床。