文丘里空化空蚀杀灭大肠杆菌的试验研究.pdf

中国环境科学 2017,37(9) ：3385~3391 China Environmental Science 文丘里空化空蚀杀灭大肠杆菌的试验研究 * 耿 坤,董志勇 ,张 凯,居文杰,赵文倩,李杨如,秦兆雨,王 磊 (浙江工业大学建筑工程学院,浙江 杭州 310014) 摘要：利用自主研发的文丘里式水力空化反应装置,选取大肠杆菌为病原微生物的指示菌,对大肠杆菌水样进行灭菌处理.使用琼脂平板计 数法检测大肠杆菌的杀灭率, 同时利用生物显微镜观察大肠杆菌在空化空蚀作用前后的形态变化.分析了文丘里管喉部长径比、初始浓度、 喉部流速、运行时间、水流空化数对大肠杆菌杀灭率的影响.实验结果表明:喉部长径比 L/R=60 时,大肠杆菌的杀灭效果最好;降低空化数、 增加喉部流速、延长运行时间、选取合适的大肠杆菌初始浓度均有利于提高大肠杆菌的杀灭率. 关键词：喉部长径比；水力空化；文丘里管；大肠杆菌；饮用水消毒 中图分类号：TV131 文献标识码：A 文章编号：1000-6923(2017)09-3385-07 Experimental study of Escherichia coli killed by hydrodynamic cavitation due to venturi tube. GENG kun, DONG * Zhi-yong , ZHANG kai, JU Wen-jie, ZHAO Wen-qian, LI Yang-ru, QIN Zhao-yu, WANG lei (College of Civil Engineering and Architecture, Zhejiang University of Technology, Hangzhou 310014, China). China Environmental Science, 2017,37(9)：3385~3391 Abstract ：A self-developed Venturi-type hydrodynamic cavitation reaction device was used. Escherichia coli was selected as the indicative bacterium of pathogenic microorganism, and water samples containing E. coli were sterilized. Killing rates of E. coli were detected by agar plate counting method. Morphological changes in E. coli before and after cavitation were observed with a biological microscope. The effects of varying throat lengths, initial concentration, throat velocity, treatment time and cavitation number on the killing rates of E. coli were analyzed. The experimental results showed that the killing effect of E. coli was best at relative throat length L/R=60. Also, lowering cavitation number, increasing throat velocity, prolonging treatment time, and choosing a suitable initial concentration of E. coli can be helpful to improving the killing rates of E. coli . Key words ：varying throat lengths ；hydrodynamic cavitation ；Venturi tube ；Escherichia coli ；drinking water disinfecti