高效液相色谱法测定亚麻纤维果胶单糖衍生条件的优化.docxVIP

研究报告高效液相色谱法测定亚麻纤维果胶单糖衍生条件的优化姜彩霞1，2 ，刘 研究报告 高效液相色谱法测定亚麻纤维果胶单糖 衍生条件的优化 姜彩霞1，2 ，刘晓兰1，2 ，田英华1，2 ，郑喜群1，2 ( 1． 齐齐哈尔大学 食品与生物工程学院，黑龙江 齐齐哈尔 161006; 2． 齐齐哈尔大学 农产品加工黑龙江省普通高校重点实验室，黑龙江 齐齐哈尔 161006) 摘 要: 采用柱前衍生高效液相色谱法( HPLC) ，利用紫外检测器对亚麻纤维果胶单糖组成进行测定分析。亚 麻纤维果胶经三氟乙酸( TFA) 水解，以 1-苯基-3-甲基-5-吡唑啉酮( PMP) 作为衍生化试剂进行柱前衍生。亚麻纤维 果胶混合单糖衍生的最佳条件为: 衍生时间 30 min，衍生温度 70 ℃ ，PMP 与单糖物质的量比为 5 ∶ 1，NaOH 与单糖 物质的量比为 3∶ 1。在此条件下测定出亚麻纤维果胶由 6 种单糖组成，其物质的量比为甘露糖∶ 鼠李糖∶ 半乳糖醛 酸∶ 葡萄糖∶ 半乳糖∶ 阿拉伯糖 = 2. 95∶ 4. 18∶ 24. 72∶ 3. 87∶ 10. 84∶ 6. 48。 关键词: 亚麻纤维果胶; PMP 柱前衍生; 高效液相色谱法; 单糖组成 中图分类号: TS102． 221 文献标志码: A 文章编号: 1003-1456( 2014) 10-0001-04 Optimization for precolumn derivatization of monosaccharide in flax fiber pectin by HPLC JIANG Cai-xia1，2 ，LIU Xiao-lan1，2 ，TIAN Ying-hua1，2 ，ZHENG Xi-qun1，2 ( 1． College of Food and Biotechnology，Qiqihar University，Qiqihar 161006，China; 2． Key Laboratory of Processing Agricultural Products of Heilongjiang Province，Qiqihar University，161006 Qiqihar，China) Abstract: High performance liquid chromatography ( HPLC ) was used for the analysis of monosaccharide present in flax fiber pectin with pre-column derivatization． Ultraviolet ( UV) detector was used to analyze for monosaccharide composition． Flax fiber pectin was hydrolyzed by trifluoroacetic acid ( TFA) ，and 1-phenyl-3-methyl-5-pyrazolone ( PMP ) was used as a derivatizing agent in pre-column derivatization． The best conditions for the pre-column derivatization were as follows : the time of the derivation reaction was 30 min，the temperature of the derivation reaction was 70 ℃ ，the molar ratio of PMP and monosaccharide was 5∶ 1，the molar ratio of NaOH and monosaccharide was 3∶ 1． Under these conditions，the results showed that flax fiber pectin consist of 6 kinds of monosaccharides，mannose， rhamnose，galacturonic acid，glucose，galactoses，arabinose with ratios of 2. 95 ∶ 4. 18 ∶ 24. 72 ∶ 3. 87 ∶ 10. 84∶ 6. 48 respectively． Key words: flax fiber pectin; HPLC; PMP pre-column derivatization; monosaccharide co