子宫肌层前列腺素F2a受体表达的调节机制研究.doc

PAGE 8 子宫肌层前列腺素F2α受体表达的调节机制研究 陈诚，常青，王琳，张利宏，梁志清作者简介：陈诚，男，湖南省澧县人， 作者简介：陈诚，男，湖南省澧县人，汉族，硕士，医师，主要从事产科危重症抢救方面的工作。发表论文2篇。电话：（023手机E-mail:chencheng961@ 通讯作者：梁志清，电话：(023收稿日期：2008-11-06；修回日期：2008- - 摘要：目的 研究子宫平滑肌前列腺素受体（PGF2α receptor，FP）孕期表达下调和临产后表达增强的分子机制。方法 对原代培养子宫平滑肌细胞用孕激素，cAMP，细胞因子及PKA化学激活剂和抑制剂处理后，采用实时RT-PCR分析子宫平滑肌细胞中FP mRNA的表达情况。结果 孕激素下调FP mRNA的表达，但cAMP对其表达无显著影响；PKA抑制剂H89促其表达。炎性细胞因子IL-1β和TNF-α促进FP mRNA的表达，IL-6降低其表达。IL-1β通过PKA途经诱导FP mRNA的表达；NFkB和C/ EBP单独或联合过度表达时可促进FP mRNA的表达。 结论 孕期FP表达下调与孕激素和cAMP相关，临产后炎性细胞因子通过PKC和NFkB途经诱导FP表达增强。 关键词：前列腺素F2α受体；孕激素；腺苷酸环化酶；细胞因子；NFkB Prostaglandin F2alpha receptor regulation in human uterine myocytes CHEN Cheng, CHANG Qing, Wang Lin，ZHANG Li-hong，LIANG Zhi-qing (Department of Gynaecology and Obstetrics, Southwest Hospital, the Third Military Medical University, Chong- qing 400038, China) Abstract：objective Study the mechanisms that are responsible for the pregnancy-associated reduction in FP mRNA expression and the later increase with the onset of labour. Methods Culture Primary human uterine myocytes cells and co- incubated the myometrial cells with progesterone, cAMP, inflammatory cytokines and different chemical activators and inhibitors, then investigated the FP mRNA expression through Quantitative RT-PCR. Results FP mRNA expression was reduced by progesterone, unaltered by cAMP (8-bromo cAMP or forskolin), but increased by the PKA antagonist H89. Interleukin (IL)-1β, tumour necrosis factor-alpha increased FP mRNA expression and IL-6 reduced FP mRNA expression. The IL-1β induced increase in FP expression was mediated at least in part via protein kinase C (PKC). NFkB and C/ EBP alone or in combination with each oher up-regulated the FP mRNA expression. Conclusions the pregnancy-induced down-regulation in myometrial FP expression is mediated by progesterone and cAMP. And that the increase with labour is induced by inflammatory cytokine activation of PKC and NFkB. Key words：F2α receptor; progester