大鼠视神经切断后视网膜Muller细胞及小胶质细胞变化特点.docVIP

大鼠视神经切断后视网膜 Muller 细胞及小胶质细胞变化特点 唐茂丹 1 ,陈家波 1 ,孙永芳 1 ,童世琼 1 ,武丽红 2 ,吴春云 2 (1 昆明医学院 05级临床医学 (眼视光学专业 ; 2 组织胚胎学教研室,云南 昆明 650031 [摘要 ]目的 观察视网膜 M uller 细胞和小胶质细胞在大鼠视神经切断后的变化特点 . 方法 应用大鼠视 神经完全横断模型,随机分为术后 1d 、 3d 、 7d 、 14d 4个损伤组和假手术组 ( 每组 n =5 ;分别用 M uller 细 胞和小胶质细胞特异性标记物谷氨酰胺合成酶 (GS 和 OX-42,进行免疫组织化学链霉菌抗生物素蛋白 -过 氧化物酶连结 (SP 法染色,以显示视网膜 M uller 细胞和小胶质细胞的变化 . 结果 在视神经切断后,大鼠视 网膜 M uller 细胞 GS 阳性产物立即增粗 、 深染,并与周围细胞紧密联系在一起, 3d 达高峰, 14d 明显减弱;小 胶质细胞 OX-42的阳性产物表达在视神经切断后 7d 明显增强, 14d 达高峰 . 结论 视神经切断后大鼠视网膜 M uller 细胞反应性增生,小胶质细胞被激活,但反应高峰晚于 M uller 细胞,其作用有待进一步研究 . [关键词 ]视神经切断; M uller 细胞;小胶质细胞 ;大鼠 [中图分类号 ]R774; R779.12[文献标识码 ]A [文章编号 ]1003-4706(2010 01-0001-05 Immunohist ochemical St udy of Muller Cells and Micr oglia Cells Responses t o Opt ic Ner ve Tr ansect ion in Rat s TANG Mao -dan 1 , CHEN Jia -bo 1 , SUN Yong -fang 1 , TONG Shi -qiong 1 , WU Li -hong 2 , WU Chun -yun 2 (1 2005Year of Clinical Medicine (Ophthalmology and Optometry ; 2 Dept. of Histology and Embryology , Kunming Medical University , Kunming Yunnan 650031, China [Abstract ]Objective To study features of the retinal M uller cells and microglia cells in rats after optic nerve transection . Methods We built the optic nerve transection model which was randomly divided into five groups :four injury groups (n =5in each group :the 1, 3, 7, and 14day groups post optic nerve injury , control group (n =5 . Then we observed the changes of M uller cells and glial cells which is immunohistochemically streptavidin-perosidase stainned by using anti-GS (glutamine synthetase and anti-OX-42antibody (amoeba-like glial cell line-specific marker . Results The expression of (GS -immunoreaction (GS-IR increased significantly compared to the control group . The most significant change occurred in the 3-day group . the expression of GS-IR had a descending tendency in the 7-day and 14-day groups compared to the 3-day group . And immunoreaction of 0X-42increased significantly compared to the control group , at day 7post-axotomy were markedly exp