光合细菌对养殖水质及凡纳滨对虾抗病力的影响.docVIP

Vol.29 No.6 2009年12月 Journal of Guangdong Ocean University Dec. 2009 第29卷 第6期 广东海洋大学学报 光合细菌对养殖水质及凡纳滨对虾抗病力的影响 陆家昌1，黄翔鹄1，李 活2，方锐林1 （1. 广东海洋大学水产学院，广东 湛江 524025；2. 茂名市金阳热带海珍养殖有限公司，广东 茂名 525000） 摘 要：在凡纳滨对虾养殖环境中人工引入不同浓度的光合细菌，监测氨氮、亚硝酸氮、化学需氧量的变化，15 d后测定凡纳滨对虾的AKP、POD、PO、SOD、抗菌、溶菌活力，以及不同光合细菌浓度下凡纳滨对虾的成活率与体重增长率，研究光合细菌对水质和凡纳滨对虾抗病力的影响。结果表明，引入光合细菌可显著降低水体化学需氧量、氨氮含量，并抑制亚硝酸盐氮的产生，提高凡纳滨对虾的抗病力。当光合细菌投放浓度为3×103 /mL时，水体中化学需氧量、氨态氮、亚硝酸盐氮含量的均值分别比对照组降低31.59%、43.42%、52.20%，差异显著（P 0.05）；对虾的AKP、POD、PO、SOD、抗菌、溶菌活力分别比照组提高31.92%、72.29%、55.56%、31.61%、39.07%、69.51%，差异显著（P 0.05）；成活率, 体重增长率分别比对照组提高18.67%、34.03%，差异显著（P 0.05）。 关键词：光合细菌；水质调控；凡纳滨对虾；抗病力 A 文章编号：1673-9159（2009）06-0087-05 中图分类号：S968.22+9 文献标志码： Effect of Photosynthetic Bacteria on Water Quality and Anti-disease Ability of Litopenaeus vannamei Xiang-hu1, LI Huo2, FANG Rui-lin1 LU Jia-chang1, HUANG (1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China；2. Maoming Sangao Fishery Development CO. Ltd. , Maoming 525000，China) Abstract: Different concentrations of Photosynthetic Bacteria were added into culture water of Litopenaeus vannamei and the concentration of NH3-N, NO2-N and COD were determined. After 15 days’ culture, the survival rate, the weight-gaining rate, the activity of AKP (alkaline phosphatase), POD (Phenoloxidase), PO (Phenoloxidase), SOD (superoxide dismutase), the anti-bacteria and bacteriolysis of the shrimp were measured. The results of comparative test between treatment and control groups showed that Photosynthetic Bacteria in appropriate density (3×103 /mL) could significantly (P0.05) improve the water quality and anti-disease ability of L. vannamei: the average concentration of NH3-N，NO2-N and COD decreased respectively by 43.42%, 52.20% and 31.59%; the survival rate and the weight-gaining rate increased respectively by 18.67% and 34.03%; the activity of anti-bacterial, bacteriolysis, AKP, PO, POD and SOD increased respectively by 39.07%, 69.51%, 31.92%, 55.56%, 72.29% and 31.61%. Key words: photosynthet