激光诱发自体荧光诊断大肠癌光谱参数分析.doc

激光诱发自体荧光诊断大肠癌光谱参数分析 ???? 激光诱发自体荧光诊断大肠癌光谱参数分析 ???? 中国肿瘤临床 2000年第7期第27卷 论著 ???? 作者：曹建彪　李世荣　高革　翟力平 ???? 单位：曹建彪(北京军区总医院消化内科　北京市　100700)；李世荣(北京军区总医院消化内科　北京市　100700)；高革(北京军区总医院消化内科　北京市　100700)；翟力平(北京军区总医院消化内科　北京市　100700) ???? 关键词：激光诱发自体荧光；大肠癌；诊断 ???? 　　摘要　目的：探讨激光诱发自体荧光(LIF)诊断大肠癌光谱参数的意义。方法：采用激光诱发自体荧光技术对61例大肠癌标本进行了检测。结果：56例(91.8%)癌标本主峰峰强度＜5500U，次峰峰强度＜2000U，波谷强度＜1800U；而相应的非癌对照组织主峰峰强度＞11000U，次峰峰强度＞4000U，波谷强度＞3800U；相差分别为1∶3.36、1∶5.94、1∶5.22。与非癌组织比较，癌组织主峰波长略偏红光，次峰略偏紫光，主、次峰间的波下降速率相对较缓。用本研究标准判别，LIF诊断大肠癌，敏感性为91.8%，特异性为100%。结论：癌组织与非癌组织的LIF有明显差异，LIF作为一种无创性内镜下恶性病变的筛检方法，用以引导内镜活检有良好的发展前景。 ???? Analysis of laser-induced Fluorescence Spectra in Colorectal Cancer Specimens ???? Cao Jianbiao　Li Shirong　Gao ge　et al(Department of Gastroenterology,Beijing Army General Hospital,Beijing) ???? 　　Abstract　Objective:To analyse the significance of laser-induced fluorecence(LIF) spectra in diagnosis of colorectal cancinoma.Methods:Sixty one cases of specimens of colorectal cancinoma were detected using the technique of LIF.Results:The positive rate was91.8%(56/61).The intensities of the first wave peak,the second wave peak and the trough in cancer specimens were＜5500u,2000u and 1800u respectively,while those in the control group were＞11000u,4000u and 3800u respectively.The ratio of cancer/control were 1∶3.36,1∶5.94 and 1∶5.22 respectively.Compared to the control group,the wave length of the first wave peak transferred to red,and the second wave peak to violet.The waves of the control group declined relatively slower than those in the cancer group,but 5 of 61 specimens had none of these typical spectra.The pathological findings revealed abundant intracellular fibrous tissue in 2,and the diagnosis of another 3 cases were not certain probably due to variation of submucosal structure.The sensitivity of LIF was91.8%,the specificity 100%.Conclusion:It is significant difference between the cancer tissue of colorectal cancer and non-cancerous tissue when examined by lIF.Therefore LIF is an ideal no