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威斯腾生物十二周年庆技术培训之脂肪间充质干细胞相关分化与炎症相关威斯腾生物技术中心目录脂肪间充质干细胞脂肪间充质干细胞分离、培养和鉴定骨关节炎和肥胖脂肪间充质干细胞分化与炎症脂肪间充质干细胞(ASCs). Adipose tissue is recognized as a complex endocrine organ that plays central roles in energy homeostasis, feeding, insulin sensitivity, and inflammation. Adipose tissue contains different cell types besides adipocytes, including adipose-derived stromal cells (ASCs). ASCs were first reported by Frohlich in 1972, as adherent, proliferating adipocyte precursors. Different names have been used to describe the adherent cell population that can be expanded from lipoaspirates, e.g. lipoblast, pericytes, preadipocytes,processed lipoaspirates (PLA) cells, and many others. Several works demonstrated the stem cell-like plasticity of ASCs and their capability of differentiating into cells of mesodermal origin, such as adipocyte, osteocyte, chondrocyte and myocyte lineages.Zuk PA, etc. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 2002; 13: 4279-4295orSVF(stromal vascular fraction)PLATo clarify nomenclature, the International Fat Applied Technology Society (IFATS) proposed to name ASCs the isolated, plastic-adherent, multipotent cell population obtained from adipose tissue through different methodsSubcutaneous fat is an abundant and accessible source of both heterogeneous stromal vascular fraction (SVF) cells and a small number of relatively homogeneous ASCs. SVF cells include vascular endothelial cells and their progenitors, smooth muscle cells, immune regulatory monocytes/macrophages and T regulatory cells.Caspar-Bauguil, S., et al., Adipose tissue lymphocytes: types and roles. J Physiol Biochem, 2009. 65(4): p. 423-36.Similarly to bone marrow mesenchymal stromal cells (BMSCs), ASCs were identifiedin vivo as cells with perivascular localization, in close contact with the other cell types.Like other MSC types, ASCs cannot be identified by a single specific marker, but multiple marker combinations are needed for the identification of the cell popul
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