心脏电生理技术基础.ppt

CELLULAR CARDIAC ELECTROPHYSIOLOGICAL TECHNIQUES;Electrical model of the membrane Standard intracellular microelectrode technique Voltage clamp technique Patch clamp technique;G=1/R;Ohm’s law;Current clamp;Intracellular microelectrode technique;;0.1 - 0.2 ?m;;;The setup;drug;Two microelectrode voltage clamp;The voltage-clamp circuit;Patch-clamp: the special case of the voltage clamp ;;;;closed open;closed open open;voltage command;Individual channels are either open or closed (no partial openings);Individual channels are either open or closed (no partial o