生物大分子分离纯化-原理与技术培训课件.pptVIP

Critical contaminants in downstream process development Contaminants Molecular weights Isoelectric points IgG Aggregates 300,000 Same as IgG Protein A 42,000 4.9-5.1 DNA 1,000-1,000,000 Highly acidic Endotoxin 100,000 Highly acidic Host cell proteins Vary Various Viruses 1,000,000 7 for most Retention of aggregates Aggregates tend to bind CHT more strongly than “monomeric” IgG. This indicates that the binding characteristics of the individual IgG molecules in an aggregate are additive. The larger the aggregate, the stronger the binding. Aggregate removal in NaCl gradients at low concentration