生物膜层干涉技术及其应用.docVIP

生物膜层干涉（BLI）技术及其应用 Biolayer Interferometry（BLI） 生物分子结合后的 BLI Biosensor ? 生物分子结合到传感器表面形成了一层生物膜 ? 生物膜对透过传感器的光波造成干涉现象 ? 干涉现象以相位移动的方式被检测，从而可以 检测结合到传感器表面分子数量的变化 Pall ForteBio解决方案 ? Label-free ? Real time ? Fluidics-free ? Fast，Accurate，Easy， Information Provided by Octet Platform Affinity characterization ? Binding KD ? On and off rates ? Rank order Specificity of binding ? Yes / No binding screens ? Epitope binning / mapping ? Isotyping Protein Concentration ? Picogram to milligram detection range Fortebio Product Portfolio Blitz Octet QKe Octet QK384 Octet RED96 Octet RED384 MW Range 分子量范围 10,000 D 5000 D 5000 D 150 D 150 D # Channels per Read 通道 1 1 - 8 1 - 16 1 - 8 1 - 16 Microplate Positions 板位 1 1 2 1 2 Biosensor Reracking 可回收 YES Yes Yes YES Ye s Robot Compatible 兼容性 No No Yes No Ye s Microplate Formats 板型 Single channel 96, 96 HA 96 / 96HA 384 / 384TW 96 96 / 96HA 384 / 384TW Read Capabilities 使用范围 Concentration, Kon, Koff, KD (Affinity)从浓度到亲和力 Sample Usage 样品使用 Non-destructive and recoverable无损害可回收 Temperature Control 温控 4°C above ambient to 40°C 仪器外形 Biosensor selection According to application sample type etc. 2012年开发了两种传感器 1. Anti-GST Biosensor: 用于含GST标签的蛋白 2. NTA Biosensor：用于含His标签的蛋白 BLI Versatility in Interaction Analysis Size 1000 nm 200 nm 75 nm Cells细胞 Bacteria细菌 Virus病毒 IgM免疫球蛋白M MW 1,000,000 Bacteria细菌 Virus病毒 Antibody – Antigen抗体-抗原 Receptor – Ligand受体-配体 IgA免疫球蛋白A DNA - DNA DNA - Protein IgG, IgD, IgE 7 nm 100,000 Antibody Fragment – Antigen - 抗体片段 抗原 Antibody Fragments 抗体片段 Antigen – Fusion Protein 融合入蛋白 Proteins 蛋白 质 Antibody – Peptide - 抗体 肽 Peptides肽 1 nm Nucleotides (DNA) Small Molecules小分 1,000 150 0.1 nm 1 子 Atoms原子 Multiple Antibody Pairings多重抗体配对 Antibody抗体 - Small Molecule Protein 蛋白- Small Molecule BLI Solution for Drug Development Wide Fit Within Discovery and Development Process Research Screening Lead Characterization Preclinical and Clinical Manufacturing and QC Assay development Small molecule or ligand screening Affinit