生物信息学课件08.pptVIP

MKMASSLAFLLLNFHVSLFLVQLLTPCSAQFSVLGPSGPILAMVGEDADLPCHLFPTM SAETMELRWVSSSLRQVVNVYADGKEVEDRQSAPYRGRTSIL * 主要发生在膜外周蛋白及膜整合蛋白胞外侧（研究较多），但有些胞质蛋白也有修饰（研究较少）。 The carbohydrate is attached to the protein in a?cotranslational?or?posttranslational modification. This process is known as?glycosylation.?Secreted extracellular proteins?are often glycosylated. In proteins that have segments extending extracellularly, the extracellular segments are also glycosylated. Glycoproteins are often important?integral membrane proteins, where they play a role in cell–cell interactions. Glycoproteins are also formed in the?cytosol, but their functions and the pathways producing these modifications in this compartment are less well understood.[2] * Select prediction programme: During our training programme N-glycosites were best predicted using BPP based methods whereas O-glycosites were best predicted using PPP method on balanced datasets. CPP method performed better on realistic datasets for O-glycosites prediction (where number of unglycosylated residues would be much higher than glycosylated residues in a sequence) Potential大于0.5认为较可能。 sequon: a consensus sequence (motif) * Potential大于0.5认为较可能。 * * 线粒体蛋白质 内质网蛋白质 高尔基复合体蛋白质 溶酶体蛋白质 过氧化物酶体蛋白质 细胞核蛋白质 * * * Dual localization: Localization names joining two abbreviated names with “ ”, such as “cyto nucl”, indicate dual localization. * * 在“Type/paste protein sequence in FASTA format”框中粘贴要预测的fasta格式的序列，选择分析类型 获得分析结果 分析方法（1）：糖蛋白预测 打开 http://www.imtech.res.in/raghava/glycoep/submit.html 选择“NetOGlyc” 软件分析O－连接糖链的连接位点 在NetOGlyc网页粘贴序列，获得分析结果 分析方法（2）：分析O－连接糖蛋白 打开/proteomics 分析方法（3）：分析N－连接糖蛋白 选择“NetNGlyc” 软件分析N－连接糖链的连接位点 在NetNGlyc网页粘贴序列，获得分析结果 打开 /proteomics （五）分析蛋白质的亚细胞定位 有助于了解： 蛋白质功能 蛋白质互作 Gene Ontology 分析蛋白质的亚细胞定位 选择“PSORT” 软件分析蛋白质在细胞中的定位() 在PSORT网页根据待分析蛋白质来源物种差异选择不同分析方法，如选择WoLF PSORT 选择物种，粘贴序列 分析结果 打开 PSORT细胞定位缩写对照表 Dual localization Protein Subcellular Localization Prediction with WoLF PSORT 其它预测蛋白质亚细胞定位工具： CELLO： .tw/ Subnuclear Compartment