以MAPK通路为靶向的肿瘤生物治疗_百度文库.doc

可能其增殖及分化能力有限 , 而适于发育学研究的 ESC 易 携带异源物质 , 培养条件要根据不同目的加以选择 。除了满 足适合 ESC 的生长需要 , 最优化的培养系统还需要其明确 组成成分 , 以便于在多个实验室重复开展 , 需要明确培养基 质 、 培养基成分 、 重组蛋白因子的种类 、 传代培养的密度与时 间 ; 另外 , ESC 的来源 、 胎胚获取方式 、 甚至培养瓶的材质等 , 这些因素都可能在一定程度对研究结果产生影响 。 参考文献 [1]　 EVANS M J, K AUF MAN M H. Establishment in culture of p luri 2 potential cells fr om mouse e mbryos [J ].Nature, 1981, 292(5819 :154-156. [2]　 THOMS ON J A, I TSK OW I TZ ELDOR J, S APH I RO S S, et al . Embryonic ste m cell lines derived fr om human blast ocysts[J ].Sci 2ence, 1998, 282(5391 :1145-1147. [3]　 HAL ME D G, KESS LER D A . F DA regulati on of ste m -cell -based therapies[J].Ne w Engl J Med, 2006, 355(16 :1730-1735. [4]　 F AN L, CROD I A N J, COLLOD I P . Culture of e mbry onic ste m cell lines fr om zebrafish[J ].Methods Cell B i ol, 2004, 76:151-160. [5]　 B I G DE L IN , ANDERSS ON M, STREHL R, et al . tati human e mbryonic ste m cells t o feeder --free 2ture conditi ons directly i 2008, 133(1 :[6]　 HE I SK ANEN A, S T, TII TI N EN S, et al . N -glycolyl 2 neura m inic acid xenoantigen conta m inati on of human e mbryonic and mesenchy mal ste m cells is substantially reversible [J ].Ste m cells, 2007, 25(1 :197-202. [7]　 K OCAOE MER A, KERN S, K LUETER H, et al . Human AB -serum as well as thr ombin -activated p latelet -rich -p las ma are suitable alternatives t o fetal calf serum f or the expansi on of mesen 2chy mal ste m cells fr om adi pose tissue [J ].Ste m cells, 2007, 25(5 :1270-1278. [8]　 AM I T M , SHAR I KI C, MARG ULETS V, et al . Feeder layer -and serum -free culture of human embryonic ste m cells[J ].B i ol Rep r od, 2004, 70(3 :837-845. [9]　 P OCHAMP ALLY R R, S M I TH J R, Y LOST ALO J, et al . Serum dep rivati on of human marr ow str omal cells (h MSCs selects f or a subpopulati on of early p r ogenit or cells with enhanced exp ressi on of OCT -4and other e mbryonic genes[J ].B l ood, 2004, 103(5 :1647-1652. [10]ZHU , CHEN J, al . Hypoxia and serum dep riva 2 ti on -in mal stem cells [J ].Ste m 24:-[]Q N J,