psicheck2质粒说明书资料.pdfVIP

T e c h n i c a l B u l l e t i n siCHECK? Vectors INSTRUCTIONS FOR USE OF PRODUCTS C8011 AND C8021. PRINTED IN USA. Revised 6/09 Part# TB329 siCHECK? Vectors All technical literature is available on the Internet at: /tbs Please visit the web site to verify that you are using the most current version of this Technical Bulletin. Please contact Promega Technical Services if you have questions on use of this system. E-mail: techserv@ 1. Description 1 2. Product Components and Storage Conditions2 3. General Considerations3 A. siCHECK? Vector Features3 B. How the siCHECK? Vectors Work4 C. Sample Experiments Using the siCHECK? Vectors6 4. siCHECK? Vector Maps 9 5. siCHECK? Vector Restriction Enzyme Tables 11 A. Restriction Enzyme Sites for the psiCHECK?-1 Vector11 B. Restriction Enzyme Sites for the psiCHECK?-2 Vector13 6. siCHECK? Vector Backbones and Components15 7. References16 8. Related Products18 1. Description (a –d) (a –f) The psiCHECK?-1 Vector (Cat.# C8011) and psiCHECK?-2 Vector (Cat.# C8021) are designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter gene. In both vectors, Renillaluciferase is used as a primary reporter gene, and the gene of interest can be cloned into the multiple cloning region located downstream of the Renillaluciferase translational stop codon. Initiation of the RNAi process toward a gene of interest results in cleavage and subsequent degradation of fusion mRNA. Measurement of d