miR-143靶向调控MMP9和LOC108635657对奶山羊乳腺细胞的作用.pdfVIP

符号说明 Amp:Ampicillin,氨苄青霉素 AP-1:Activatorprotein-1,活化蛋白-1 AP-2:Activatorprotein -2,活化蛋白-2 APS:Ammoniumpersulfate,过硫酸铵 BMECs:Bovinemammary epithelialcells,奶牛乳腺上皮细胞 Bp:Basepair,碱基对 cDNA:Complementary deoxyribonucleic acid,互补DNA CDS:Coding sequence,编码区序列 ceRNA:competingendogenousRNA,竞争内源性RNA DMSO:Dimethylsulfoxide,二甲基亚砜 E.coli:Escherichiacoli,大肠杆菌 FBS:Fetalbovineserum,胎牛血清 GAPDH:Glyceraldehyde-phosphatedehydrogenase,磷酸甘油醛脱氢酶 GMECs:Dairy goat Mammary Epithelial Cells,奶山羊乳腺上皮细胞 GnRH:Gonadotropin-releasinghormone,促性腺激素释放激素 GO:GeneOntology ,基因本体论 KEGG:KyotoEncyclopediaofGenesand Genomes,京都基因与基因组百科全书 miRNA:microRNA ,微小RNA MMP2:Matrixmetalloprotein-2,基质金属蛋白酶-2 MMP9:Matrixmetalloprotein-9,基质金属蛋白酶-9 MRE:Metalresponseelement ,金属应答元件 mRNA:MessengerribonucleicAcid,信使核糖核酸 ncRNA:non-codingRNA,非编码RNA OD:Opticaldensity ,光密度 PBS:Phosphatebuffered saline,磷酸盐缓冲液 RT-PCR:Reversetranscription-polymerasechain reaction,逆转录聚合酶链反应 SCC:Somaticcell count ,牛乳体细胞数 SDS:Sodium dodecyl sulfate ,十二烷基磺酸钠 TEMED:Tetramethyl diethylamine,四甲基二乙胺 目 录 中文摘要·····································································································I ABSTRACT······························································································III 1 前言········································································································1 1.1崂山奶山羊的特点及泌乳性能·····································································1 1.1.1崂山奶山羊的特征··················································································1 1.1.2 崂山奶山羊的泌乳性能············································································1 1.2miRNAs 的研究进展················································································· 1 1.2.1miRNAs 的概述····················································································· 1 1.2.2miRNAs对乳腺的调控···························